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品种反应性 主要应用 宿主 格式 抗体类型 Mk, H, M IH(P) Rb Purified Polyclonal Antibody 描述 产品目录编号 AB5405 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Opsin Antibody, Red/Green 背景信息 The full range of color discrimination in humans is based on the presence and function of three cone photoreceptor mechanisms. Each cone type possesses a photo-sensitive pigment-protein complex consisting of 11-cis retinal and a unique opsin protein, which gives sensitivity in the short (S cone, peak sensitivity about 420nm), middle (M cone, peak sensitivity about 530nm with polymorphism; Winderckx et al., 1993; Neitz & Neitz, 1998), and long (L cone, peak sensitivity about 560nm with polymorphism; Neitz & Jacobs, 1990) wavelengths of the light spectrum. All three opsins are transmembrane proteins with seven membrane-spanning regions. Genes for the three types of cone opsins and the rod photoreceptor rhodopsin gene seem to be homologous with varying amounts of conservation. Strongest conservation is between the middle (green) and long (red) wavelength sensitive pigments on the X chromosome, suggesting a relatively recent duplication/divergence event (Nathans, 1989; Nathans et al., 1992). The S cone (blue) opsin is located on chromosome 7 and seems to have stronger conservation with rhodopsin. Cone photoreceptor distribution in humans is dominated by the M and L cone pigments. 产品信息 格式 Purified 控制 - Retina
演示 Purified rabbit polyclonal in buffer containing PBS, 0.02 M phosphate, 0.25 M NaCl, pH 7.6 with 0.1% sodium azide. 应用 应用 Anti-Opsin Antibody, Red/Green detects level of Opsin & has been published & validated for use in IH(P). 主要应用 - Immunohistochemistry (Paraffin)
应用说明 Immunohistochemistry:
1:200-1:300 on formalin-fixed, paraffin-embedded mouse retina tissue.
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Recombinant human red/green opsin. 表位 Red/Green 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recognizes opsin, red/green. 品种反应性 MonkeyHumanMouse Species Reactivity Note Mouse. Reactivity with other species has not been determined 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_020061.3
Entrez基因汇总 This gene encodes for a light absorbing visual pigment of the opsin gene family. The encoded protein is called red cone photopigment or long-wavelength sensitive opsin. Opsins are G-protein coupled receptors with seven transmembrane domains, an N-terminal extracellular domain, and a C-terminal cytoplasmic domain. This gene and the medium-wavelength opsin gene are tandemly arrayed on the X chromosome and frequent unequal recombination and gene conversion may occur between these sequences. X chromosomes may have fusions of the medium- and long-wavelength opsin genes or may have more than one copy of these genes. Defects in this gene are the cause of partial, protanopic colorblindness. 基因符号 - RCP
- OPN1LW
- CBP
- CBBM
纯化方法 Protein A chromatography UniProt编号 - P04000
UniProt汇总 FUNCTION: SwissProt: P04000 # Visual pigments are the light-absorbing molecules that mediate vision. They consist of an apoprotein, opsin, covalently linked to cis-retinal.
SIZE: 364 amino acids; 40572 Da
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: The three color pigments are found in the cone photoreceptor cells.
PTM: Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.
DISEASE:SwissProt: P04000 # Defects in OPN1LW are the cause of partial colorblindness protan series (CBP) [MIM:303900]; also called protanopia.SIMILARITY:SwissProt: P04000 ## Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.产品使用声明 质量保证 Immunohistochemistry(paraffin) Analysis:
Opsin (cat. #: AB5405) staining pattern in mouse eye. Tissue pretreated with Citrate, pH 6.0. This lot of antibody was diluted to 1:200, using IHC-Select® Detection with HRP-DAB. Immunoreactivity is seen restricted to the cone shape cells responsible for photoreception.
Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Mouse Eye使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for up to 6 months at 2-8°C in undiluted from date of receipt. Do not freeze. Keep vials well sealed to prevent evaporation. 包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,05-886R,Anti-phospho-STAT5A/B (Tyr694/699) Antibody, clone 94-10C-9-10C-2, rabbit monoclonal
下一件merck millipore产品：merck millipore,默克密理博,219474,Cathepsin G Substrate IV, Fluorogenic - Calbiochem
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