merck millipore,默克密理博,AB5228-50UL,Anti-Potassium Channel MaxiK Antibody, calcium activated, aa1097-1196

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merck millipore,默克密理博,AB5228-50UL,Anti-Potassium Channel MaxiK Antibody, calcium activated, aa1097-1196
产品名称：Anti-Potassium Channel MaxiK Antibody, calcium activated, aa1097-1196
产品型号：AB5228-50UL
Anti-Potassium Channel MaxiK Antibody, calcium activated, aa1097-1196 detects level of Potassium Channel MaxiK & has been published & validated for use in WB.

merck millipore,默克密理博,AB5228-50UL,Anti-Potassium Channel MaxiK Antibody, calcium activated, aa1097-1196

产品介绍

merck millipore,默克密理博,AB5228-50UL,Anti-Potassium Channel MaxiK Antibody, calcium activated, aa1097-1196

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R	WB	Rb	Affinity Purified	Polyclonal Antibody

描述
产品目录编号	AB5228-50UL
品牌系列	Chemicon®
商名	Chemicon
描述	Anti-Potassium Channel MaxiK Antibody, calcium activated, aa1097-1196
Alternate Names	KCa1.1 BKCa Slo KCNMA1

产品信息
格式	Affinity Purified
控制	CONTROL ANTIGEN: Included free of charge with the antibody is 120 μg of control antigen (lyophilized powder in phosphate buffered saline, pH 7.4, containing 5% sucrose and 0.025% sodium azide). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For positive control, in Western blotting, use 20 ng protein per lane. For negative control, preincubate 3-5 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
演示	Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.025% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).

应用
应用	Anti-Potassium Channel MaxiK Antibody, calcium activated, aa1097-1196 detects level of Potassium Channel MaxiK & has been published & validated for use in WB.
主要应用	Western Blotting
应用说明	Western blot: 1-1.5 μg/mL (1:200:1:300) using ECL Dilutions should be made using a carrier protein such as BSA (1-3%). Typical bands detected are ~125kDa in rat membrane preparations. Excellent methods given in Ling, et al (2003) Optimal working dilutions must be determined by the end user.

生物信息
免疫原品种	A purified fusion protein (GST)1 and a C-terminal part (residues 1097-1196) of mouse Slo (mSlo) alpha subunit of BKCa channel.
表位	C-terminal sequence SHSSHSSQ
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	Corresponds to the C-terminus of the longer form of BKCa channel a subunit and overlaps by >40 residues with a shorter form.
品种反应性	Rat
抗体类型	Polyclonal Antibody
Entrez基因编号	NM_002247.2 NM_001014797.1
Entrez基因汇总	MaxiK channels are large conductance, voltage and calcium-sensitive potassium channels which are fundamental to the control of smooth muscle tone and neuronal excitability. MaxiK channels can be formed by 2 subunits: the pore-forming alpha subunit, which is the product of this gene, and the modulatory beta subunit. Intracellular calcium regulates the physical association between the alpha and beta subunits. Alternatively spliced transcript variants encoding different isoforms have been identified.
基因符号	OTTHUMP00000064159 KCNMA1 OTTHUMP00000064161 OTTHUMP00000060198 KCNMA K OTTHUMP00000064158 MaxiK VCAlpha) SLO-ALPHA MGC71881 OTTHUMP00000064157 SAKCA OTTHUMP00000064156 OTTHUMP00000064164 Slo1 BKTM OTTHUMP00000064165 hSlo mSLO1 OTTHUMP00000064154 Slo-alpha SLO OTTHUMP00000064162 KCa1.1 OTTHUMP00000064155 OTTHUMP00000064160 DKFZp686K1437
UniProt编号	Q08460-2
UniProt汇总	FUNCTION: SwissProt: Q12791 # Potassium channel activated by both membrane depolarization or increase in cytosolic Ca(2+) that mediates export of K(+). It is also activated by the concentration of cytosolic Mg(2+). Its activation dampens the excitatory events that elevate the cytosolic Ca(2+) concentration and/or depolarize the cell membrane. It therefore contributes to repolarization of the membrane potential. Plays a key role in controlling excitability in a number of systems, such as regulation of the contraction of smooth muscle, the tuning of hair cells in the cochlea, regulation of transmitter release, and innate immunity. In smooth muscles, its activation by high level of Ca(2+), caused by ryanodine receptors in the sarcoplasmic reticulum, regulates the membrane potential. In cochlea cells, its number and kinetic properties partly determine the characteristic frequency of each hair cell and thereby helps to establish a tonotopic map. Kinetics of KCNMA1 channels are determined by alternative splicing, phosphorylation status and its combination with modulating beta subunits. Highly sensitive to both iberiotoxin (IbTx) and charybdotoxin (CTX). SIZE: 1236 amino acids; 137560 Da SUBUNIT: Homotetramer; which constitutes the calcium-activated potassium channel. Interacts with beta subunits KCNMB1, KCNMB2, KCNMB3 and KCNMB4. Beta subunits are accessory, and modulate its activity. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Widely expressed. Except in myocytes, it is almost ubiquitously expressed. DOMAIN: SwissProt: Q12791 The S0 segment is essential for the modulation by the accessory beta subunits KCNMB1, KCNMB2, KCNMB3 and KCNMB4. & The S4 segment, which is characterized by a series of positively charged amino acids at every third position, is part of the voltage-sensor. & The pore-forming domain (also referred as P region) is imbedded into the membrane, and forms the selectivity filter of the pore. It contains the signature sequence of potassium channels that displays selectivity to potassium. & The RCK N-terminal domain mediates the homotetramerization, thereby promoting the assembly of monomers into functional potassium channel. It includes binding sites for Ca(2+) and Mg(2+) (By similarity). & The calcium bowl constitutes one of the Ca(2+) sensors and probably acts as a Ca(2+)-binding site. There are however other Ca(2+) sensors regions required for activation of the channel. PTM: Phosphorylated (Probable). Phosphorylation by kinases such as PKA and/or PKG. In smooth muscles, phosphorylation affects its activity. DISEASE: SwissProt: Q12791 # Defects in KCNMA1 are the cause of generalized epilepsy and paroxysmal dyskinesia (GEPD) [MIM:609446]. Epilepsy is one of the most common and debilitating neurological disorders, affecting more than 40 million people worldwide. Paroxysmal dyskinesias are another heterogeneous group of neurological disorders characterized by sudden, unpredictable, disabling attacks of involuntary movement often requiring life-long treatment. The coexistence of epilepsy and paroxysmal dyskinesia in the same individual or family is an increasingly recognized phenomenon. The basic pathophysiology underlying the coexistence of these two disorders is caused by mutations in the KCNMA1 gene. SIMILARITY: Belongs to the potassium channel family. Calcium- activated subfamily. & Contains 1 RCK N-terminal domain. MISCELLANEOUS: The protein was initially thought to contain two functionally distinct parts: The core channel (from the N-terminus to the S9 segment) that mediates the channel activity, and the cytoplasmic tail (from the S9 segment to the C-terminus) that mediates the calcium sensing. The situation is however more complex, since the core channel also contains binding sites for Ca(2+) and Mg(2+).

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Maintain lyophilized material at -20°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.