merck millipore,默克密理博,AB3038,Anti-Uncoupling Protein 1 Antibody

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merck millipore,默克密理博,AB3038,Anti-Uncoupling Protein 1 Antibody
产品名称：Anti-Uncoupling Protein 1 Antibody
产品型号：AB3038
Anti-Uncoupling Protein 1 Antibody detects level of Uncoupling Protein 1 & has been published & validated for use in ELISA & WB.

merck millipore,默克密理博,AB3038,Anti-Uncoupling Protein 1 Antibody

产品介绍

merck millipore,默克密理博,AB3038,Anti-Uncoupling Protein 1 Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
M	WB, ELISA	Rb	Affinity Purified	Polyclonal Antibody

描述
产品目录编号	AB3038
品牌系列	Chemicon®
商名	Chemicon
描述	Anti-Uncoupling Protein 1 Antibody
Alternate Names	UCP1

产品信息
格式	Affinity Purified
演示	Affinity purified immunoglobulin. Liquid in PBS with 0.1% BSA.

应用
应用	Anti-Uncoupling Protein 1 Antibody detects level of Uncoupling Protein 1 & has been published & validated for use in ELISA & WB.
主要应用	Western Blotting ELISA
应用说明	Western Blotting: 1-10 μg/mL using Chemiluminescence technique. An antibody made to this epitope has detected approx. 32 kDa protein in brown adipose tissues (Feil & Rafael 1994). Immunohistochemistry: we recommend the use of the antibody at 5-10 mg/mL on formaldehyde fixed tissue. ELISA: (1:10,000-1:100,000; using 50-100 ng UCP1 control peptide (Catalog Number AG382) /well. Optimal working dilutions must be determined by end user.

生物信息
免疫原品种	19 aa peptide sequence (designated UCP12; cytoplasmic) at the C-terminus of the mouse/rat UCP1 (Bouillaud et al. 1986; Ridley et al. 1986; Kozak et al. 1988; Cassard et al. 1990; Miroux et al. 1993). The peptide has no significant homology with UCP2 or UCP3. This peptide is available for blocking as catalog number AG382.
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	Uncoupling Protein 1 (UCP1), mouse. Mouse/rat UCP1 is a 307 amino acid mitochondrial uncoupling protein (Bouillaud et al. 1986; Ridley et al. 1986; Kozak et al. 1988; Cassard et al. 1990; Miroux et al. 1993). It is only 59% homologous with UCP2 (Fleury et al. 1997). Human UCP1 is located on chromosome 4 (Bouillaud et al. 1986; Ridley et al. 1986; Kozak et al. 1988; Cassard et al. 1990; Miroux et al. 1993). UCP1 is primarily expressed in brown adipose tissues. SPECIES REACTIVITIES: The antibody has not yet been tested for cross-reactivity to other species. The sequence used for immunization shows 100% homology to rat, 94% with golden hamster, 88% with porcine, and 78% with rabbit, human and canine UCP1.
品种反应性	Mouse
抗体类型	Polyclonal Antibody
Entrez基因编号	NM_021833.3
Entrez基因汇总	Mitochondrial uncoupling proteins (UCP) are members of the family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed only in brown adipose tissue, a specialized tissue which functions to produce heat.
基因符号	SLC25A7 UCP1 Thermogenin UCP
UniProt编号	P25874
UniProt汇总	FUNCTION: SwissProt: P25874 # UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat. SIZE: 307 amino acids; 33005 Da SUBUNIT: Acts as a dimer forming a proton channel. SUBCELLULAR LOCATION: Mitochondrion inner membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Brown adipose tissue. SIMILARITY: SwissProt: P25874 ## Belongs to the mitochondrial carrier family. & Contains 3 Solcar repeats.

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For antibodies with volumes of 200 μl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.

储存和货运信息

存储条件

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For antibodies with volumes of 200 μl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.