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  • merck millipore,默克密理博,AB5158,Anti-Calcium Channel Antibody, Voltage Gated α 1D

  • merck millipore,默克密理博,AB5158,Anti-Calcium Channel Antibody, Voltage Gated α 1D

    产品名称：Anti-Calcium Channel Antibody, Voltage Gated α 1D
    产品型号：AB5158
    Anti-Calcium Channel Antibody, Voltage Gated α 1D is an antibody against Calcium Channel for use in IH, IP & WB.

    merck millipore,默克密理博,AB5158,Anti-Calcium Channel Antibody, Voltage Gated α 1D

  • 产品介绍
  • merck millipore,默克密理博,AB5158,Anti-Calcium Channel Antibody, Voltage Gated α 1D

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    R, MWB, IHCRbAffinity PurifiedPolyclonal Antibody
    描述
    产品目录编号 AB5158
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-Calcium Channel Antibody, Voltage Gated α 1D
    Alternate Names
    • L-type
    背景信息 Voltage-sensitive calcium channels (vscc) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1c gives rise to l-type calcium currents. Long-lasting (l-type) calcium channels belong to the "high-voltage activated" (hva) group. They are blocked by dihydropyridines (dhp), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-aga-IIIA). They are however insensitive to omega-conotoxin- gvia (omega-ctx-gvia) and omega-agatoxin-IVA (omega-aga-IVA) (Swiss Prot).
    产品信息
    格式 Affinity Purified
    控制
    • CONTROL ANTIGEN: Included free of charge with the antibody is 20 μg of control antigen (lyophilized powder in phosphate buffered saline, pH 7.4, containing 5% sucrose and 0.025% sodium azide). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
    演示 Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.05% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
    应用
    应用 Anti-Calcium Channel Antibody, Voltage Gated α 1D is an antibody against Calcium Channel for use in IH, IP & WB.
    主要应用
    • Western Blotting
    • Immunohistochemistry
    应用说明 In preparing full-length protein samples for Western blot or immunoprecipitation, the procedures should be performed at 4°C, with the following protease inhibitor mixture: pepstatin A (1 μg/mL), leupeptin (1 μg/mL), aprotinin (1 μg/mL), Pefabloc SC (0.2 mM), benzamidine (0.1 mg/mL), and calpain inhibitors I and II (8 μg/mL each).

    Western blot: 1:200 using ECL on rat membrane preperations. LMW form shows a 190kDa band while the HMW form shows a 210kDa band.

    Immunohistochemistry on rat brain sections.

    Dilutions should be made using a carrier protein such as BSA (1-3%).

    Optimal working dilutions and protocols must be determined by the end user.
    生物信息
    免疫原品种 Purified peptide CND1 from 859-875 of alpha 1D subunit of rat brain voltage-gated calcium channel (Accession P27732).
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Rabbit
    特异性 Recognizes all forms of alpha 1D subunit from the voltage-gated calcium channel. Does not cross react with any other calcium channel antigens tested so far. Sequence conservation: hamster and mouse (16/17); human (15/17); chicken (12/17) residues identical.
    品种反应性 RatMouse
    抗体类型 Polyclonal Antibody
    Entrez基因编号
    • NM_000720.1
    基因符号
    • CACNL1A2
    • CACNA1D
    • CCHL1A2
    • CACH3
    • Cav1.3
    • CACN4
    UniProt编号
    • Q01668
    UniProt汇总 FUNCTION: SwissProt: Q01668 # Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1D gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the high-voltage activated (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin- GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA).
    SIZE: 2161 amino acids; 245141 Da
    SUBUNIT: Voltage-dependent calcium channels are multisubunit complexes, consisting of alpha-1, alpha-2, beta and delta subunits in a 1:1:1:1 ratio. The channel activity is directed by the pore- forming and voltage-sensitive alpha-1 subunit. In many cases, this subunit is sufficient to generate voltage-sensitive calcium channel activity. The auxiliary subunits beta and alpha-2/delta linked by a disulfide bridge regulate the channel activity. Interacts with RIMBP2 (By similarity).
    SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
    TISSUE SPECIFICITY: Expressed in pancreatic islets and in brain, where it has been seen in hippocampus, basal ganglia, habenula and thalamus. No expression in skeletal muscle.
    DOMAIN: SwissProt: Q01668 Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.
    SIMILARITY: Belongs to the calcium channel alpha-1 subunit family.
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
    包装信息
    数量 200 µL

    merck millipore,默克密理博,AB5158,Anti-Calcium Channel Antibody, Voltage Gated α 1D

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