merck millipore,默克密理博,04-1116,Anti-beta Actin Antibody, clone EP1123Y, rabbit monoclonal

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merck millipore,默克密理博,04-1116,Anti-beta Actin Antibody, clone EP1123Y, rabbit monoclonal
产品名称：Anti-beta Actin Antibody, clone EP1123Y, rabbit monoclonal
产品型号：04-1116
Anti-beta Actin Antibody, clone EP1123Y, Rabbit detects level of beta Actin & has been published & validated for use in IH(P), WB & IC.

merck millipore,默克密理博,04-1116,Anti-beta Actin Antibody, clone EP1123Y, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-1116,Anti-beta Actin Antibody, clone EP1123Y, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	ICC, WB	Rb	Unpurified	Monoclonal Antibody

描述
产品目录编号	04-1116
描述	Anti-beta Actin Antibody, clone EP1123Y, rabbit monoclonal
Alternate Names	beta cytoskeletal actin PS1TP5-binding protein 1 actin, beta actin, cytoplasmic 1 beta actin
背景信息	Actin is a 43kDa highly conserved structural protein. There are three main actin isotypes (alpha, beta and gamma) which show > 90% amino-acid (aa) homology between isotypes and > 98% homology within members of a particular isotypic group. The majority of the isotype heterogeneity is located in the amino-terminal 30 aa. The different actin isotypes have been shown to behave very differently in vitro and in vivo. Recent studies describe differential subcellular localization of gamma-actin and isotype specific binding of actin associated proteins, (Pardo, 1983, Otey, 1986, Bassell, 1998). Beta actin is one of the two nonmuscle cytoskeletal actins and is relatively stable and appears to remain at normal levels regardless of experimental treatment. It is generally used as an internal control for experiments.

产品信息
格式	Unpurified
控制	T47D and HeLa cell lysates.
演示	Rabbit Monoclonal in buffer containing 50mM Tris-Glycine (pH 7.4), 0.15M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Anti-beta Actin Antibody, clone EP1123Y, Rabbit detects level of beta Actin & has been published & validated for use in IH(P), WB & IC.
主要应用	Immunocytochemistry Western Blotting
应用说明	Immunohistochemistry Analysis: 1:50 dilution from a previous lot detected beta Actin in human muscle tissue. Immunocytochemistry: 1:50 dilution from a previous lot was used on IC.

生物信息
免疫原品种	Synthetic peptide corresponding to residues near the C-terminus of human beta Actin.
表位	C-terminus
克隆	EP1123Y
宿主	Rabbit
特异性	This antibody recognizes beta Actin.
同种型	IgG
品种反应性	RatHumanMouse
Species Reactivity Note	Human, mouse, and rat.
抗体类型	Monoclonal Antibody
Entrez基因编号	P60709
基因符号	PS1TP5BP1 Beta-actin
纯化方法	Unpurified
UniProt编号	P60709
UniProt汇总	FUNCTION: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. SUBUNIT STRUCTURE: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57 SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Found in a complex with XPO6, Ran, ACTB and PFN1. Component of the MLL5-L complex, at least composed of MLL5, STK38, PPP1CA, PPP1CB, PPP1CC, HCFC1, ACTB and OGT. Interacts with XPO6. SUBCELLULAR LOCATION: Cytoplasm › cytoskeleton. INVOLVEMENT IN DISEASE: Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss. MISCELLANEOUS: In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility. SEQUENCE SIMILARITIES: Belongs to the actin family.

产品使用声明
质量保证	Evaluated by Western Blot on T47D and HeLa cell lysates. Western Blot Analysis: 1:500 dilution of this antibody was used to detect beta Actin in T47D and HeLa cell lysates.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.