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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, M IHC, ICC Rb Purified Polyclonal Antibody 描述 产品目录编号 AB5026 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Enkephalin Antibody, Met 产品信息 格式 Purified 演示 Protein A/G purified immunoglobulin. Liquid containing 0.1% sodium azide. 应用 应用 Anti-Enkephalin Antibody, Met is an antibody against Enkephalin for use in IC, IH. 主要应用 - Immunohistochemistry
- Immunocytochemistry
不建议的应用 - Immunohistochemistry (Paraffin)
应用说明 Immunohistochemistry: 2-10μg/mL. See suggested portocols. Optimal working dilutions must be determined by the end user.
IMMUNOHISTOCHEMISTRY
Anesthetize animals and perfuse them transcardially as follows*:
a) Flush with cold (4°C) oxygenated Calcium free Tyrodes
b) Perfuse with 4% formaldehyde in 0.16M phosphate buffer at pH 6.9
c) Perfuse with 10% sucrose solution in 0.1M phosphate buffer (pH 7.2)
* For better visualization of cell bodies immunoreactive for Met5-enkephalin, pretreatment with colchicine 24-48 hours before perfusion may be required (see reference 2)
Sections
Cut 5-30 mm sections using a cryostat and mount them on subbed histological slides.
Immunofluorescence
Dilute Met-enkephalin antibody with 0.1M phosphate buffered saline (PBS, pH 7.4) containing 1% BSA and 0.01% Triton X-100. Incubate sections overnight at 2-8°C and then wash in PBS (three times for 10 minutes). Incubate for one hour at room temperature with properly diluted donkey anti-rabbit secondary antibody conjugated to a fluorescent probe such as FITC (Catalog Number AP182F), Rhodamine (Catalog Number AB182R) or Cy3 (Catalog Number AP182C). Wash in PBS (three times for 10 minutes) and mount with a PBS/glycerol solution containing 0.1% phenylenediamine to reduce fading. If not stained with FITC, sections can be dehydrated in grading alcohols (50%, 75%, 80%, 96% and 100%), cleared with xylene and mounted with DPX (Fluka, Ronkonkoma, NY). Staining can be visualized by using both conventional and confocal microscopy.
Indirect Avidin-Biotin Peroxidase
Incubate sections with 0.3% H2O2 in PBS for 15 minutes at room temperature to block endogenous peroxidase. Rinse sections with PBS (three times for 10 minutes). Dilute Met-enkephalin antibody with 0.1M phosphate buffered saline (PBS, pH 7.4) containing 1% BSA and 0.01% Triton X-100. Incubate sections overnight at 2-8°C and then wash in PBS (three times for 10 minutes). Incubate sections with properly diluted biotinilated goat anti-rabbit secondary antibody (Catalog Number AP132B) diluted in PBS (do not add sodium azide!) for one hour at room temperature. Rinse sections three times for 15 minutes and incubate sections with horseradish peroxidase-streptavidin complex (Catalog Number SA108) properly diluted in PBS/0.1% Triton X-100 for 40 minutes at room temperature. Wash sections three times for 15 minutes in PBS and incubate them with 0.03% of diaminobenzidine (DAB) in PBS/0.015% H2O2 for 3-15 minutes at room temperature. Dehydrate sections in grading alcohols (50%, 75%, 80%, 96% and 100%), clean them in xylene and coverslip with DPX or Permount. Sections can be examined using conventional microscopy.
Important Note: During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For antibodies with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial on a tabletop centrifuge to dislodge any liquid in the container's cap.生物信息 免疫原品种 Synthetic Met5-enkephalin peptide (H-Tyr-Gly-Gly-Phe-Met-OH) conjugated through the N-terminal tyrosine to BSA. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Met5-enkephalin. Cross reactivity: Leu5-enkephalin 5.8%, beta-endorphin <0.01% and beta-Lipotropin <0.01%. 品种反应性 RatHumanMouse 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_006211.2
基因符号 - proenkephalin
- PENK
UniProt编号 - P01210
UniProt汇总 FUNCTION: SwissProt: P01210 # Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress.
SIZE: 267 amino acids; 30787 Da
SUBCELLULAR LOCATION: Secreted.
PTM: The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.
SIMILARITY: SwissProt: P01210 ## Belongs to the opioid neuropeptide precursor family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to six months after date of receipt. Avoid repeated freeze/thaw cycles. 包装信息 数量 50 µg
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