merck millipore,默克密理博,AB3553,Anti-Thiazide-Sensitive NaCl Cotransporter Antibody

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Merck Millipore 默克密理愽

品种反应性	主要应用	宿主	格式	抗体类型
Rb, H, M, R	WB, IHC	Rb	Affinity Purified	Polyclonal Antibody

描述
产品目录编号	AB3553
Replaces	AB3564P
品牌系列	Chemicon®
商名	Chemicon
描述	Anti-Thiazide-Sensitive NaCl Cotransporter Antibody

产品信息
格式	Affinity Purified
控制	POSITIVE CONTROL: Rat kidney.
演示	Affinity Purified immunoglobulin in PBS containing no preservatives.

应用
应用	This Anti-Thiazide-Sensitive NaCl Cotransporter Antibody is validated for use in IH, WB for the detection of Thiazide-Sensitive NaCl Cotransporter.
主要应用	Western Blotting Immunohistochemistry
应用说明	Immunoblotting: 1:2,000 using rat kidney extract. The antibody reacts with the 160-190 kDa protein (the molecular weight varies depending on conditions for protein extraction/electrophoresis). The suggested dilution and blocking buffer is 150 mM NaCl, 10 mM Tris, pH 7.4 containing 5% low fat milk and 0.04% Tween. Suggested transfer membrane is PVDF or Nylon. Suggested gel percentage is 7%. Overnight incubation with the antibody at 2-8°C is recommended. Immunohistochemistry: 1:500-1:2,000 on rat or mouse kidney tissue. Suggested fixative is 3% paraformaldehyde for 5 minutes by vascular perfusion. Suggested blocking buffer is PBS containing 10% normal goat serum. Suggested dilution buffer is PBS containing 1% BSA. Overnight incubation with the antibody at 2-8°C is recommended.Optimal working dilutions must be determined by the end user.

生物信息
免疫原品种	Synthetic peptide from the N-terminal of human/rat/mouse NCC.
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	Recognizes the renal thiazide-sensitive NaCl cotransporter (NCC). The antibody stains the apical plasma membrane and subapical membrane vesicles in renal distal convoluted tubule cells.
品种反应性	RabbitHumanMouseRat
抗体类型	Polyclonal Antibody
Entrez基因编号	NM_000339.1
基因符号	TSC SLC12A3 NCCT
纯化方法	Immunoaffinity purified
UniProt编号	P55017
UniProt汇总	FUNCTION: SwissProt: P55017 # Electrically silent transporter system. Mediates sodium and chloride reabsorption. SIZE: 1021 amino acids; 113139 Da SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Predominant in kidney. DISEASE: SwissProt: P55017 # Defects in SLC12A3 are the cause of Gitelman syndrome (GS) [MIM:263800]. GS is an autosomal recessive disease characterized by diverse abnormalities in electrolyte homeostasis including hypokalaemic metabolic alkalosis. GS is a subset of Bartter syndrome. SIMILARITY: SwissProt: P55017 ## Belongs to the SLC12A transporter family.

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

包装信息
数量	50 µL

merck millipore,默克密理博,AB3553,Anti-Thiazide-Sensitive NaCl Cotransporter Antibody

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