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品种反应性 主要应用 宿主 格式 抗体类型 M, H ICC, WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB2989 描述 Anti-Metadherin Antibody Alternate Names - astrocyte elevated gene-1
- Astrocyte elevated gene-1 protein
- Lysine-rich CEACAM1 co-isolated protein
- Metastasis adhesion protein
背景信息 Metadherin is a type 2 transmembrane protein who's overexpression was first described in breast cancers. It plays an important role in the metastasis of breast cancers into the lungs as a secondary site of development. Antibodies reactive to the lung-homing domain of metadherin and siRNA-mediated knockdown of metadherin expression in breast cancer cells inhibited experimental lung metastasis, indicating that tumor cell metadherin mediates localization at the metastatic site (Brown, 2009). 产品信息 格式 Affinity Purified 控制 - HeLa cell lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. 应用 应用 Anti-Metadherin Antibody is an antibody against Metadherin for use in WB & IC. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Western blot Analysis: 0.25 µg/ml of this antibody detected Metadherin in 10 µg of HEK293, HepG2, and Jurkat cell lysates.
Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected Metadherin in NIH/3T3 and HeLa cells.生物信息 免疫原品种 Recombinant GST-tagged proteins corresponding at or around the lung homing domain of mouse Metadherin. 表位 Homing domain 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 The antibody recognizes Metadherin at and around the homing domain. 同种型 IgG 品种反应性 MouseHuman Species Reactivity Note Demonstrated to react with human and mouse. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_080278.3
基因符号 - LYRIC/3D3
- MTDH
- 3D3
- 3D3/LYRIC
- AEG-1
- AEG1
- LYRIC
纯化方法 Affinity Purfied UniProt编号 - Q80WJ7
UniProt汇总 FUNCTION: Downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance By similarity.
SUBUNIT STRUCTURE: Interacts with BCIPP, CREBBP/CBP and RELA/p65 By similarity.
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass membrane protein By similarity. Nucleus membrane; Single-pass membrane protein By similarity. Cell junction › tight junction By similarity. Nucleus › nucleolus by similarity. Cytoplasm › perinuclear region By similarity. Note: In epithelial cells, recruited to tight junctions (TJ) during the maturation of the TJ complexes. A nucleolar staining may be due to nuclear targeting of an isoform lacking the transmembrane domain. TNF-alpha causes translocation from the cytoplasm to the nucleus By similarity.
TISSUE SPECIFICITY: In the mammary gland, expressed at the apical surface of epithelial cells lining ducts, as well as in the mammary fat pad. Not detected in the spleen, kidney, lung, or skin; minute amounts seen in the liver. Expressed in Purkinje neurons in the early postnatal and adult cerebellum. Overexpressed in mammary tumors (at protein level).
CAUTION: Was originally (Ref.1) thought to be a type II membrane protein but this is inconsistent with the results of multiple phosphorylation studies because this topology would locate the phosphorylation sites in the lumen or extracellularly rather than in the cytoplasm.产品使用声明 质量保证 Evaluated by Western blot in HeLa cell lysate.
Western blot Analysis: 0.25 µg/ml of this antibody detected Metadherin in 10 µg of HeLa cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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