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品种反应性 主要应用 宿主 格式 抗体类型 H WB, ELISA Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB3226 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Uncoupling Protein 2 Antibody Alternate Names - UCP2
产品信息 格式 Affinity Purified 演示 Affinity purified immunoglobulin. Liquid in 100mM Tris, pH 7.5 with 0.2% BSA as stabilizer and 0.05% sodium azide. 应用 应用 Detect Uncoupling Protein 2 using this Anti-Uncoupling Protein 2 Antibody validated for use in ELISA & WB. 主要应用 - Western Blotting
- ELISA
应用说明 Western Blotting: 1-10 μg/mL using Chemiluminescence technique.
Immunohistochemistry: Has not been tested. It is recommended that you use 2-20 μg/mL on formaldehyde fixed tissue.
ELISA: 1:10,000-1:100,000; using 50-100 ng UCP2 control peptide/well.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 13 aa peptide sequence near the 3rd and 4th transmembrane domain of human UCP2 (Fleury et al. 1997; Gimeon et al. 1997). The peptide has no significant homology with UCP1, UCP3 or UCP4 (Boss et al. 1997). 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Uncoupling Protein 2 (UCP2), human. Human UCP2 is a 309 amino acid mitochondrial uncoupling protein (Fleury et al. 1997; Gimeon et al. 1997). It is only 59% homologous with UCP1 found in brown adipose tissues. UCP2 has wide tissue distribution in tissues (brain, kidney, liver, brown adipose tissue, heart, and muscle). UCP2 may play a critical role in energy balance, body weight, and thermoregulation (Fleury et al. 1997; Gimeon et al. 1997; Flier & Lowell 1997).
SPECIES REACTIVITIES: The antibody has not yet been tested for cross-reactivity to other species. The sequence used for immunization shows 100% homology to mouse and rat UCP2 and 92% homology with canine, porcine and bovine.品种反应性 Human 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_003355.2
Entrez基因汇总 Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed in many tissues, with the greatest expression in skeletal muscle. It is thought to play a role in nonshivering thermogenesis, obesity and diabetes. Chromosomal order is 5'-UCP3-UCP2-3'. 基因符号 - SLC25A8
- UCP2
- UCPH
UniProt编号 - P55851
UniProt汇总 FUNCTION: SwissProt: P55851 # UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat.
SIZE: 309 amino acids; 33229 Da
SUBUNIT: Acts as a dimer forming a proton channel (By similarity).
SUBCELLULAR LOCATION: Mitochondrion inner membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Widely expressed in adult human tissues, including tissues rich in macrophages. Most expressed in white adipose tissue and skeletal muscle.
SIMILARITY: SwissProt: P55851 ## Belongs to the mitochondrial carrier family. & Contains 3 Solcar repeats.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. 包装信息 数量 50 µg
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