merck millipore,默克密理愽
  • merck millipore,默克密理博,ABT170,Anti-alpha Tubulin Antibody, nontyrosinated

  • merck millipore,默克密理博,ABT170,Anti-alpha Tubulin Antibody, nontyrosinated

    产品名称：Anti-alpha Tubulin Antibody, nontyrosinated
    产品型号：ABT170
    Detect Tubulin using this rabbit polyclonal antibody, Anti-alpha Tubulin Antibody, nontyrosinated validated for use in western blotting, ELISA & ICC.

    merck millipore,默克密理博,ABT170,Anti-alpha Tubulin Antibody, nontyrosinated

  • 产品介绍
  • merck millipore,默克密理博,ABT170,Anti-alpha Tubulin Antibody, nontyrosinated

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    PorcineICC, WB, ELISARbSerumPolyclonal Antibody
    描述
    产品目录编号 ABT170
    Replaces 04-1117
    描述 Anti-alpha Tubulin Antibody, nontyrosinated
    Alternate Names
    • Tubulin alpha-3 chain
    • Tubulin alpha-1A chain
    • Alpha-tubulin 3
    • Tubulin B-alpha-1
    背景信息 Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase adds a C-terminal tyrosin to monomeric alpha tubulin. Assembled microtubules can be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.
    产品信息
    格式 Serum
    演示 Rabbit polyclonal serum in buffer containing serum with 0.05% sodium azide.
    应用
    应用 Detect Tubulin using this rabbit polyclonal antibody, Anti-alpha Tubulin Antibody, nontyrosinated validated for use in western blotting, ELISA & ICC.
    主要应用
    • Immunocytochemistry
    • Western Blotting
    • ELISA
    应用说明 Western Blotting Analysis: A representative lot from an independent laboratory specifically detected nontyrosinated alpha Tubulin and not tyrosinated alpha Tubulin from purified porcine alpha Tubulin (Gundersen, G. G., et al. (1984). Cell. 38(3):779-89.).

    ELISA Analysis: A representative lot from an independent laboratory specifically detected nontyrosinated alpha Tubulin, and not nontyrosinated alpha Tubulin in an indirect ELISA and in a competitive ELISA (Gundersen, G. G., et al. (1984). Cell. 38(3):779-89.).

    Immunocytochemistry Analysis: A representative lot from an independent laboratory specifically detected nontyrosinated alpha Tubulin, and not nontyrosinated alpha Tubulin in TC-7 cells in interphase (Gundersen, G. G., et al. (1984). Cell. 38(3):779-89.).
    生物信息
    免疫原品种 KLH-conjugated linear peptide corresponding to human nontyrosinated alpha Tubulin.
    宿主 Rabbit
    特异性 This antibody detects the nontyrosinated form of alpha Tubulin.
    品种反应性 Porcine
    Species Reactivity Note Demonstrated to react with Mouse and Porcine. Predicted to react with Human and Rat based on 100% sequence homology.
    抗体类型 Polyclonal Antibody
    Entrez基因编号
    • NP_001257328
    基因符号
    • TUBA3
    • TUBA1A
    纯化方法 Unpurified
    UniProt编号
    • Q71U36
    产品使用声明
    质量保证 Evaluated by Western Blotting in NIH/3T3 cell lysate, which were untreated or treated with Pancreatic Carboxypeptidase (PCA).

    Western Blotting Analysis: A 1:2,000 dilution of this antibody detected alpha Tubulin, nontyrosinated in 10 µg of PCA treated NIH/3T3 cell lysate and demonstrated a loss of signal in untreated NIH/3T3 cell lysate.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at -20°C from date of receipt.
    Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
    包装信息
    数量 100 µL

    merck millipore,默克密理博,ABT170,Anti-alpha Tubulin Antibody, nontyrosinated

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