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品种反应性 主要应用 宿主 格式 抗体类型 M, H ICC, WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB2999 描述 Anti-BNIP3 Antibody Alternate Names - BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
- BCL2/adenovirus E1B 19kDa interacting protein 3
- BCL2/adenovirus E1B 19kD-interacting protein 3
背景信息 BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 (BNIP3) is a member of the NIP3 family, a singular group of death-inducing, mitochondrial proteins. BNIP3 is comprised of several domains of interest; a highly conserved domain (CD), a C-terminal transmembrane (TM) domain, and a BH3 domain. Strongly upregulated by hypoxia, BNIP3 induces a caspase-independent cell death characterized by mitochondrial damage. Overexpression has been observed in the brain after stroke and in the adult heart following myocardial infarction. Recent studies have shown BNIP3 to be involved in cell growth inhibition through suppression of the mTOR pathway and that, when localized to the nucleus, it has the paradoxical capability of repressing apoptosis in tumors by binding the AIF expression gene. 产品信息 格式 Affinity Purified 控制 - Etoposide-treated and untreated PC3 cell lysates
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-BNIP3 Antibody detects level of BNIP3 & has been published & validated for use in WB & IC. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Immunocytochemistry Analysis: A previous lot detected BNIP3 in human glioblastoma-astrocytoma epithelial-like cells. (courtesy of Spencer Gibson, Manitoba Institute of Cell Biology.) 生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to an internal region of human BNIP3. 表位 Transmembrane domain 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 品种反应性 MouseHuman Species Reactivity Note Demonstrated to react with human. Predicted to react with mouse based on 100% sequence homology. Other homologies: Rat, equine, and ox (90% sequence homology). 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_004043
Entrez基因汇总 This gene is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein which is responsible for the protection of virally-induced cell death, as well as E1B 19 kDa-like sequences of BCL2, also an apoptotic protector. This gene contains a BH3 domain and a transmembrane domain, which have been associated with pro-apoptotic function. The dimeric mitochondrial protein encoded by this gene is known to induce apoptosis, even in the presence of BCL2. [provided by RefSeq]. 基因符号 - Nip3
- BNIP3
- NIP3
纯化方法 Affinity purified UniProt编号 - Q12983
UniProt汇总 FUNCTION: Apoptosis-inducing protein that, which can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2.
SUBUNIT STRUCTURE: Homodimer. Binds to BCL2. Interacts with BNIP3L. Also can interact with adenovirus E1B 19 kDa protein or Epstein-Barr virus BHRF1.
SUBCELLULAR LOCATION: Mitochondrion membrane; Single-pass membrane protein Potential. Note: Coexpression with the EIB 19-kDa protein results in a shift in NIP3 localization pattern to the nuclear envelope.
SEQUENCE SIMILARITIES: Belongs to the NIP3 family.产品使用声明 质量保证 Evaluated by Western Blot in etoposide-treated and untreated PC3 cell lysates.
Western Blot Analysis: 0.5 µg/mL of the antibody detected BNIP3 in 10 µg of etoposide-treated and untreated PC3 cell lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 50 µg
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