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品种反应性 主要应用 宿主 格式 抗体类型 Mk, H, M, R, Op, B, Pm WB Rb Purified Polyclonal Antibody 描述 产品目录编号 AB2298 描述 Anti-BMAL1 Antibody Alternate Names - bHLH-PAS protein JAP3
- Aryl hydrocarbon receptor nuclear translocator-like protein 1
- Brain and muscle ARNT-like 1
- Member of PAS protein 3
- Basic-helix-loop-helix-PAS protein MOP3
背景信息 Brain and muscle Arnt-like protein-1 (BMAL1; also known as MOP3 or Arnt3) is a transcription factor known to regulate circadian rhythm. BMAL1 is the only component of the mammalian circadian clock whose sole deletion in a mouse model generates arrhythmicity. In addition to defects in the clock, these BMAL1 null-mice also have reproductive problems are small in stature, age quickly and have progressive arthropathy that results in having less overall locomotor activity than wild type mice. Recent phenotyping data suggests that BMAL1 and its partner Clock also play a role in regulation of glucose homeostasis and metabolism. Finally, BMAL1, NPAS2, and PER2 have been associated with seasonal affective disorder in humans. BMAL1 transcription is circadian and reciprocally regulated by NR1D1 (Rev-erb-alpha) and RORA, which establishes a second interlocking loop in the mammalian circadian clock. 产品信息 格式 Purified 控制 - HeLa nuclear extract tissue lysate.
演示 Purified rabbit polyclonal antibody in 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3. 应用 应用 This Anti-BMAL1 Antibody is validated for use in WB for the detection of BMAL1. 主要应用 - Western Blotting
生物信息 免疫原品种 KLH conjugated synthetic linear peptide corresponding to the internal domain of BMAL1. 表位 Internal Domain 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Cat. # AB2298 recognizes the internal domain of BMAL1. 品种反应性 MonkeyHumanMouseRatOpossumBovinePrimate Species Reactivity Note Human. May react with mouse, rat, opossum, bovine, primate and monkey. Based on sequence homology. Reactivity with other species has not been determined. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_001075390.1
纯化方法 Antigen affinity purified UniProt编号 - O00327
UniProt汇总 Function: ARNTL-CLOCK heterodimers activate E-box element (3'-CACGTG-5') transcription of a number of proteins of the circadian clock. This transcription is inhibited in a feedback loop by PER, and also by CRY proteins By similarity.
Subunit structure: Component of the circadian clock oscillator which includes the CRY proteins, CLOCK or NPAS2, ARNTL or ARNTL2, CSNK1D and/or CSNK1E, TIMELESS and the PER proteins. Efficient DNA binding requires dimerization with another bHLH protein. Heterodimerization with CLOCK is required for E-box-dependent transactivation, for CLOCK nuclear translocation and degradation, and, for phosphorylation of both CLOCK and ARNTL. Interaction with PER and CRY proteins requires translocation to the nucleus. Interaction of the CLOCK-ARNTL heterodimer with PER or CRY inhibits transcription activation. Interacts with HSP90; with AHR in vitro, but not in vivo By similarity.
Subcellular location: Nucleus
Post-translational modification: Acetylated on Lys-544 upon dimerization with CLOCK. Acetylation facilitates CRY1- mediated repression.
Phosphorylated upon dimerization with CLOCK. Ref.5 Ref.6
Sumoylated on Lys-266 upon dimerization with CLOCK. Ref.7
Sequence similarities: Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains.产品使用声明 质量保证 Western Blot: 1:5000 dilution detected BMAL1 on 10 μg of HeLa nuclear extract. 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8ºC from date of receipt. 包装信息 数量 100 µg
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