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merck millipore,默克密理博,ST1202,Anti-PGC-1α Mouse mAb (4C1.3)
产品名称：Anti-PGC-1α Mouse mAb (4C1.3)
产品型号：ST1202
This Anti-PGC-1α Mouse mAb (4C1.3) is validated for use in Immunoblotting, Immunocytochemistry, Immunoprecipitation, Paraffin Sections for the detection of PGC-1α.
merck millipore,默克密理博,ST1202,Anti-PGC-1α Mouse mAb (4C1.3)
- 产品介绍
重要规格表
宿主 M 描述 概述 Recognizes the endogenous forms of PGC-1α, which includes the ~113 kDa PGC-1α protein and the ~38 kDa splice variant, in brown adipose tissue, liver, and kidney. 产品目录编号 ST1202 品牌系列 Calbiochem®
同义词 Anti-Peroxisome Proliferator-Activated Receptor- γ Coactivator 1 α 应用数据 
Detection of human PGC-1α by immunoblottiong. Samples: Whole cell lysates from COS cells transformed with wild-type PGC-1α (5 µg, lane 1; 10 µg, lane 2; 20 µg, lane 3), whole cell extract from brown adipose tissue of a cold-exposed mouse (lane 4), and whole cell lysate from CHO cells transformed with NT-PGC-1α (0.5 µg, lane 5). Primary antibody: Anti-PGC-1α Mouse mAb (4C1.3) (Cat. No. ST1202) (1 µg/ml). Detection chemiluminescence.
Detection of mouse PGC-1α by immunoblottiong. Samples: NT-PGC-1α Positive Control, (lane 1), whole cell extract from brown adipose tissue of mice kept at room temperature (50 µg; lanes 2-4), whole cell extract from brown adipose tissue of mice exposed to 4°C for 5 h (50 µg; lanes 5-7), and PGC-1α positive control (lane 8). Primary antibody: Anti-PGC-1α Mouse mAb (4C1.3) (Cat. No. ST1202) (1 µg/ml). Detection: chemiluminescence.
Detection of mouse PGC-1α by immunoblottiong. Samples: PGC-1α positive control (lane 1), nuclear extract from mouse liver (75 µg, lane 2), whole cell extract from mouse kidney (100 µg, lane 3), and whole cell extract from kidney of a PGC-1α knock-out mouse (100 µg, lane 4). Primary antibody: Anti-PGC-1α Mouse mAb (4C1.3) (Cat. No. ST1202) (1 µg/ml). Detection: chemiluminescence.参考 参考 Lai, L., et al. 2008. Genes Dev. 14, 1948.
Rodgers, J.T., et al. 2008. FEBS Lett. 582, 46.
Mazzucotelli, A., et al. 2007. Diabetes 10, 2467.
Nemoto, S., et al. 2005. J. Biol. Chem. 16, 16456.产品信息 形式 Liquid 配方 One vial of 100 µg antibody in 50 mM PBS, see vial for lot-specific concentration. One vial of PGC-1α Positive Control and one vial of NT-PGC-1α Positive Control, supposed as 25 µl whole cell extracts in RIPA buffer containing 10 mM &beta-mercaptoethanol and 1% SDS; load 10 µl per lane; add sample buffer prior to SDS-PAGE loading. 防腐剂 None 应用 应用参考 Paraffin Sections
Koh, Y.J., et al. 2009. Exp. Mol. Med. 41, 880.主要应用 Immunoblotting (Western Blotting)
Immunocytochemistry
Immunoprecipitation
Paraffin Sections
生物信息 免疫原 a recombinant protein consisting of amino acids 1-120 of mouse PGC-1α 免疫原品种 Mouse 克隆 4C1.3 宿主 Mouse 同种型 IgG 品种 human, mouse, rat 安全信息 R相 R: 21/22-36/37/38
Harmful in contact with skin and if swallowed.
Irritating to eyes, respiratory system and skin.S相 S: 26-36/37-45
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing and gloves.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).储存和货运信息 货运代码 Dry Ice Only
毒性 Multiple Toxicity Values, refer to MSDS
储存 ≤ -70°C
避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
特殊指南 Following initial thaw, aliquot and freeze (-70°C).
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