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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, M WB, FC, ICC M FITC Monoclonal Antibody 描述 产品目录编号 MAB4212F 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-HU-B27 Antibody, FITC conjugated Alternate Names - Com-B27
背景信息 HU-B27 (HLA- Com-B27) is involved in the presentation of foreign antigens to the immune system. HLA B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta 2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. 产品信息 格式 FITC 控制 - Control IgG2a-FITC (MABC002F) as an isotype control
演示 The reagent is supplied in 1.0 mL phosphate-buffered saline, pH 7.4, containing 0.2% bovine serum albumin and 0.1% sodium azide. Purified from mouse ascitic fluid. The characteristics of each lot are tested by gel electrophoresis and flow cytometry. 应用 应用 Detect HU-B27d using this Anti-HU-B27 Antibody, FITC conjugated validated for use in FC, IC, IF & WB. 主要应用 - Western Blotting
- Flow Cytometry
- Immunocytochemistry
应用说明 Flow cytometry and Immunofluorescence: use 10 mL direct from the vial per 100 mL of whole blood, or 1 x 106 peripheral blood mononuclear cells (PBMC) in 100 mL buffer.
Flow cytometric analysis of lymphocyte staining for HLA-B27 gave the following results:
Subjects/Fluorescence Intensity
Heterozygous HLA-B27+/Strong
Homozygous HLA-B7+/Weak
Heterozygous HLA-B7+/Weak - negative
Non B27, Non B7/Negative
This reagent is useful in the investigation of HLA-B27 in diseases such as ankylosing spondylitis.
SUGGESTED USAGE:
Flow cytometry and immunofluorescence - use 10 µl direct from the vial per 100 µl of whole blood, or 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 µl buffer.
Use Control IgG2a-FITC (MABC002F) as an isotype control.
Please note that this reagent should be used in a single-colour single-step assay only.生物信息 宿主 Mouse 特异性 This reagent binds strongly to the HLA-B27 antigen. It also binds weakly to the HLAB7 antigen. This formulation demonstrates less cross reactivity to HLA-B7 than Chemicon products, MAB1285 and MAB1285F. 同种型 IgG2a 品种反应性 RatHumanMouse Species Reactivity Note Human, mouse and rat. 抗体类型 Monoclonal Antibody 基因符号 - GCCD2
- MRAP
- C21orf61
- FGD2
- FALP
- B27
UniProt编号 - P03989
产品使用声明 质量保证 Evaluated by Western Blot on Jurkat lysates.
Western Blotting Analysis:
1:500 dilution of this antibody detected Com-B27 on 10 µg of Jurkat lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 6 months at 2-8ºC in the dark from date of receipt. 包装信息 数量 1 mL
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下一件merck millipore产品：merck millipore,默克密理博,PC686,Anti-PUMA (Ab-1) (2-16) Rabbit pAb
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