merck millipore,默克密理博,MABN494,Anti-Fig4/Sac3 Antibody, clone N202/7

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Merck Millipore 默克密理愽

品种反应性	主要应用	宿主	格式	抗体类型
R, M	WB	M	Purified	Monoclonal Antibody

描述
产品目录编号	MABN494
描述	Anti-Fig4/Sac3 Antibody, clone N202/7
Alternate Names	SAC domain-containing protein 3 Polyphosphoinositide phosphatase Phosphatidylinositol 3,5-bisphosphate 5-phosphatase
背景信息	Fig4/Sac3 phosphatase, a 97-kDa protein, specifically removes the 5-phosphate from the inositol ring of PI(3,5)P2, a membrane-bound phospholipid, which acts as a molecular signal for trafficking and fusion of intracellular vesicles. It is broadly expressed in all eukaryotic cells and plays a vital role in intracellular membrane trafficking and signaling. Mutations in Fig4/Sac3 have been linked to pale tremor mice that exhibit neuropathy with severe central and peripheral neuronal degeneration, including the loss of neurons in the dorsal root ganglion and the sciatic nerve. Fig4/Sac3 knockout mice have been shown to be smaller in size and exhibit abnormal tremor and gait and neuronal autophagy. Fig4/Sac3 deficiency is also reported to cause accumulation of cytoplasmic vacuoles in tissues, cultured fibroblasts, and neurons. Mutations in Fig4/Sac3 have been reported in patients with amyotrophic lateral sclerosis (ALS) and Charcot-Marie-Tooth Disease 4J that is characterized by severe motor dysfunction.

产品信息
格式	Purified
控制	Neuro-2a cell lysate
演示	Purified mouse monoclonal IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

应用
应用	This Fig4/Sac3 antibody is validated for use in WB for the detection of the Fig4/Sac3 protein.
主要应用	Western Blotting
应用说明	Western Blot Analysis: A representative lot from an independent laboratory detected Fif4/Sac3 in adult rat brain membrane and in WT mouse brain lysate, and demonstrated a loss of signal in Fif4/Sac3 knockout mouse brain lysate. Western Blot Analysis: A representative lot from an independent laboratory detected Fig4/Sac3 in mouse brain and in P4 spinal chord protein extracts (Yan, Q., et al. (2012). J Neurol Sci. 312(1-2):102-107.).

生物信息
免疫原品种	Recombinant protein corresponding to the C-terminus of mouse Fig4/Sac3.
表位	C-terminus
克隆	N202/7
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Mouse
特异性	This antibody recognizes the C-terminus of Fig4.
同种型	IgG1κ
品种反应性	RatMouse
Species Reactivity Note	Demonstrated to react with Mouse and Rat. Other Homologies: Human (91% sequence homology).
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_598760
基因符号	Sac3 Fig4
纯化方法	Protein G purified
UniProt编号	Q91WF7

产品使用声明
质量保证	Evaluated by Western Blot in Neuro-2a cell lysate. Western Blot Analysis: 1 µg/mL of this antibody detected Fig4/Sac3 in 10 µg of Neuro-2a cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at 2-8°C from date of receipt.

包装信息
数量	100 µg

merck millipore,默克密理博,MABN494,Anti-Fig4/Sac3 Antibody, clone N202/7

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