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品种反应性 主要应用 宿主 格式 抗体类型 R, H, M WB M Purified Monoclonal Antibody 描述 产品目录编号 04-804 品牌系列 Upstate
商名 - Upstate
描述 Anti-NFAT1 Antibody, clone 8G4.2 Alternate Names - nuclear factor of activated T-cells, cytoplasmic,<br />calcineurin-dependent 2
- nuclear factor of activated T-cells, preexisting component 2 <br />preexisting nuclear factor of activated T-cells 2
- NFAT pre-existing subunit
- NFAT transcription complex, preexisting component
- T cell transcription factor NFAT1
- nuclear factor of activated T-cells, cytoplasmic
- cytoplasmic
- cytoplasmic,<br />calcineurin-dependent 2
背景信息 NFAT (Nuclear Factor of Activated T-cells) is a family of transcription factors that are located in the cytoplasm in their inactive state. NFAT translocates to the nucleus upon stimulation of the T-cell receptor where it plays a key role in gene expression in immune response. NFAT is dephosphorylated and activated by the phosphatase PP2B/Calcineurin. This results in its nuclear localization and transcriptional activity. For NFAT1 (NFATC2, nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2), calcineurin is known to target the region between residues 114-126. Mutations in this region of NFAT1 result in its inability to de-phosphorylate and subsequent nuclear translocation. Upon activation, NFAT mediates the transcriptional induction of cytokines and other immuno-regulatory genes during immune responses, and is expressed in T-cells, B-cells, mast cells and NK cells. 产品信息 格式 Purified 演示 Purified mouse monoclonal in PBS with 0.05% NaN3. 应用 应用 This Anti-NFAT1 Antibody, clone 8G4.2 is validated for use in WB, IF for the detection of NFAT1. 主要应用 - Western Blotting
生物信息 免疫原品种 GST-fusion protein corresponding to amino acids 1-297 of human NFAT1. 表位 N-term 克隆 8G4.2 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Detects NFAT1. 同种型 IgMκ 品种反应性 RatHumanMouse Species Reactivity Note Human, Mouse, and Rat. Other species have not been tested. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_001129493.1
Entrez基因汇总 This gene is a member of the nuclear factor of activated T cells (NFAT) family. The product of this gene is a DNA-binding protein with a REL-homology region (RHR) and an NFAT-homology region (NHR). This protein is present in the cytosol and only translocates to the nucleus upon T cell receptor (TCR) stimulation, where it becomes a member of the nuclear factors of activated T cells transcription complex. This complex plays a central role in inducing gene transcription during the immune response. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq] 基因符号 - NFATp
- KIAA0611
- NF-ATP
- NF-ATp
- NFAT1
- NFATP
纯化方法 Ammonium sulfate precipitation UniProt编号 - Q13469
UniProt汇总 FUNCTION: Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF.
SUBUNIT: Member of the multicomponent NFATC transcription complex that consists of at least two components, a pre-existing cytoplasmic component NFATC2 and an inducible nuclear component NFATC1. Other members such as NFATC4, NFATC3 or members of the activating protein-1 family, MAF, GATA4 and Cbp/p300 can also bind the complex. The phosphorylated form specifically interacts with XPO1; which mediates nuclear export. NFATC proteins bind to DNA as monomers. Interacts with NFATC2IP (By similarity).
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC play a key role in the gene transcription.
ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative splicing. Additional isoforms seem to exist.
TISSUE SPECIFICITY: Expressed in thymus, spleen, heart, testis, brain, placenta, muscle and pancreas.
INDUCTION: Inducibly expressed in T-lymphocytes upon activation of the T-cell receptor (TCR) complex. Induced after co-addition of phorbol 12-myristate 13-acetate (PMA) and ionomycin.
DOMAIN: Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors (By similarity).
PTM: In resting cells, phosphorylated by NFATC-kinase on at least 18 sites in the 99-363 region. Upon cell stimulation, all these sites except Ser-243 are dephosphorylated by calcineurin. Dephosphorylation induces a conformational change that simultaneously exposes an NLS and masks an NES, which results in nuclear localization. Simultaneously, Ser-53 or Ser-56 is phosphorylated; which is required for full transcriptional activity.
SIMILARITY: Contains 1 RHD (Rel-like) domain.产品使用声明 质量保证 Routinely evaluated by Western Blot 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
包装信息 数量 100 µg
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