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品种反应性 主要应用 宿主 格式 抗体类型 R, B, H, M WB Rb Purified Polyclonal Antibody 描述 产品目录编号 AB19167 Replaces 04-1048 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-MMP-2 Antibody, whole molecule Alternate Names - neutrophil gelatinase
- 72 kDa gelatinase
- 72kD type IV collagenase
- Gelatinase A
- Matrix metalloproteinase-2
- collagenase type IV-A
- matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)
- matrix metalloproteinase 2
- matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IV collagenase)
- matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)
- matrix metalloproteinase-II
- 72kD gelatinase
- 72kD type IV collagenase)
- matrix metalloproteinase-II
背景信息 MMPs have a common mode of activation, a conserved amino acid sequence in the putative metal-binding active site region and are inhibited by specific tissue inhibitors of metalloproteinases (TIMPs). These MMPs and TIMPs could be expressed by either the cancer of the stromal cells. There is a co-operation between tumor and stromal cells, in particular for the production of 72-kD type IV collagenase, involved in the disruption of basement membranes. A lack of TIMP-1 expression from invasive cancer cells could also contribute to matrix destruction. 产品信息 格式 Purified 控制 - Positive control: Conditioned, serum-free medium from TPA-treated human fetal lung (HFL-1) cells. Placenta or bladder, breast, or ovarian carcinomas.
演示 Purified rabbit polyclonal in buffer containing 10 mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide. 应用 应用 Detect MMP-2 using this Anti-MMP-2 Antibody, whole molecule validated for use in IH(P) & WB. 主要应用 - Western Blotting
应用说明 Immunohistochemistry (formalin/paraffin):
10-20 µg/mL of a previous lot was kept for 30 min at RT. No special pretreatment is required for staining formalin-fixed paraffin-embedded sections.
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 A synthetic peptide from the second half of human MMP-2. 表位 Whole molecule 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 AB19167 recognizes protein at 72 kDa, identified as matrix metalloproteinase 2. MMP-2 is also known as 72 kDa collagenase IV or gelatinase A. It is synthesized as a 631 amino acid proenzyme that is activated by cleavage of the first 80 amino acids. AB19167 shows no cross-reaction with (pro) and active forms of other MMPs. Cytoplasmic localization. 品种反应性 RatBovineHumanMouse Species Reactivity Note Bovine and Rat. Expected to react with human and mouse based on sequence homology. Reactivity with other species has not been confirmed. 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_004530.2
Entrez基因汇总 Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades type IV collagen, the major structural component of basement membranes. The enzyme plays a role in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. 基因符号 - TBE-1
- CLG4
- CLG4A
- EC 3.4.24.24
- MMP-2
- MMP-II
- MONA
纯化方法 Protein A chromatography UniProt编号 - P08253
UniProt汇总 FUNCTION: SwissProt: P08253 # In addition to gelatin and collagens, it cleaves KiSS1 at a Gly- -Leu bond.
COFACTOR: Binds 4 calcium ions per subunit. & Binds 2 zinc ions per subunit.
SIZE: 660 amino acids; 73882 Da
SUBUNIT: Ligand for integrin alpha-V/beta-3.
TISSUE SPECIFICITY: Produced by normal skin fibroblasts.
DOMAIN: SwissProt: P08253 The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
PTM: The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT- MMP3).
DISEASE: SwissProt: P08253 # Defects in MMP2 are the cause of multicentric osteolysis nodulosis and arthropathy (MONA) [MIM:605156]. Inherited osteolyses or 'vanishing bone' syndromes are rare disorders of unknown etiology characterized by destruction and resorption of affected bones. MONA is an autosomal recessive osteolysis with multicentric involvement characterized by carpal and tarsal resorption, crippling arthritic changes, marked osteoporosis, palmar and plantar subcutaneous nodules and distinctive facies. & Defects in MMP2 are the cause of Winchester syndrome [MIM:277950]. Winchester syndrome is an autosomal recessive osteolysis syndrome. Winchester syndrome is severe with generalized osteolysis and osteopenia. Subcutaneous nodules are usually absent. Winchester syndrome has been associated with a number of additional features including coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy. However, these features are not always present and have occasionally been observed in other osteolysis syndromes. The clinical and molecular findings suggest that Winchester syndrome and MONA are allelic disorders that form a continuous clinical spectrum.
SIMILARITY: Belongs to the peptidase M10A family. & Contains 3 fibronectin type-II domains. & Contains 4 hemopexin-like domains.产品使用声明 质量保证 Routinely evaluated by Western Blot on PC12 lysates.
Western Blot Analysis: 1:500 dilution of this lot detected MMP-2 on 10 µg of PC12 lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8ºC from date of receipt. 包装信息 数量 100 µg
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