- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
Replacement Information Replacement Information 05-904 is a recommended replacement for 07-221 重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Vrt WB, ICC M Purified Monoclonal Antibody 描述 产品目录编号 05-904 Replaces 07-221 品牌系列 Upstate
商名 - Upstate
描述 Anti-HA Tag Antibody 概述 Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. 背景信息 Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. 产品信息 格式 Purified 控制 - RIPA lysates from HEK293 cells.
演示 Purified mouse monoclonal IgG3 in buffer containing PBS, pH 7.4 containing 0.05% sodium azide. 应用 应用 Detect HA Tag using this Anti-HA Tag Antibody validated for use in IC, IP & WB. 主要应用 - Western Blotting
- Immunocytochemistry
应用说明 Immunoprecipitation:
5 μg/mL of his antibody has been reported by an independent laboratory to immunoprecipitate recombinant HA-tagged fusion protein.
Immunocytochemistry:
5 μg/mL of this antibody has been reported by an independent laboratory to immunostain HA-tagged fusion protein.生物信息 免疫原品种 KLH-conjugated, synthetic peptide corresponding to amino acids 98-106 of influenza hemaglutinin. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Recognizes HA tagged proteins overexpressed in cells, including both amino or carboxy-termini of targeted proteins in transfected mammalian cells. 同种型 IgG3 品种反应性 Vertebrates Species Reactivity Note Most common vertebrate species tested. Reactivity with other species has not been confirmed. 抗体类型 Monoclonal Antibody 纯化方法 Protein A purfied 产品使用声明 质量保证 Routinely evaluated by western blot of recombinant protein containing the HA-tag at the amino terminus in RIPA lysates from HEK293 cells.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected a recombinant protein containing the HA-tag at the amino terminus in RIPA lysates from HEK293 cells.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 200 µg
上一件merck millipore产品：merck millipore,默克密理博,HTS008RTA,Ready-to-Assay™ CCR3 Chemokine Receptor Frozen Cells
下一件merck millipore产品：merck millipore,默克密理博,MABE159,Anti-Fox1 Antibody, clone D8F8
相关产品
merck millipore,默克密理博,SF-414,CDKN2A; Human, Cyanine 5 RNA Detection Probe,SmartFlare
merck millipore,默克密理博,CBL66,Anti-Atrial Natriuretic Peptide Antibody, clone 23/1
merck millipore,默克密理博,ABC324,Anti-PDL-1 Antibody
merck millipore,默克密理博,476480,PKC 20-28, Cell-Permeable, Myristoylated - Calbiochem
merck millipore,默克密理博,HTS008RTA,Ready-to-Assay™ CCR3 Chemokine Receptor Frozen Cells
merck millipore,默克密理博,MABE159,Anti-Fox1 Antibody, clone D8F8
merck millipore,默克密理博,AB2242,Anti-SUR1/2 Antibody
merck millipore,默克密理博,MABC777,Anti-ING1, clone EPR6788, Rabbit Monoclonal Antibody
merck millipore,默克密理博,AB19167,Anti-MMP-2 Antibody, whole molecule
merck millipore,默克密理博,MAB5206,Anti-Amyloid Antibody, β 1-16, clone DE2