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品种反应性 主要应用 宿主 格式 抗体类型 M, H WB Rb Purified Polyclonal Antibody 描述 产品目录编号 AB16941 Replaces 04-579 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Apaf-1 Antibody, NT Alternate Names - Apoptosis Protease-activating Factor 1
产品信息 格式 Purified 演示 Affinity purified immunoglobulin. Liquid in PBS containing 0.02% sodium azide. 应用 应用 Anti-Apaf-1 Antibody, N-terminus is an antibody against Apaf-1 for use in WB. 主要应用 - Western Blotting
应用说明 Western blot: 1:1000 to 1:4000
Whole cell lysate from HeLa cells can be used as a positive control and a 130 kDa band should be detected. Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Polyclonal antibody raised against a peptide corresponding to amino acids 12 to 28 of human Apaf1 (Zou et al. 1997) The sequences of the immunogenic peptide are identical between human and mouse (Zou et al. 1997; Cecconi et al. 1998). 表位 N-terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Apoptosis is related to many diseases and is induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and was designated Apaf1 for apoptosis protease-activating factor 1(Zou et al. 1997; Cecconi et al. 1998). Apaf1 binds to cytochrome C (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 which is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis (Li et al. 1997). Apaf1 can also associate with caspase-4 and caspase-8 (Hu et al. 1998). Apaf1 is ubiquitously expressed in human tissues (Zou et al. 1997). 品种反应性 MouseHuman 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_181869.1
- NM_001160.2
- NM_013229.2
- NM_181861.1
- NM_181868.1
Entrez基因汇总 This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. 基因符号 - CED4
- APAF1
- DKFZp781B1145
- Apaf-1
- KIAA0413
UniProt编号 - O14727
UniProt汇总 FUNCTION: SwissProt: O14727 # Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.
SIZE: 1248 amino acids; 141840 Da
SUBUNIT: Monomer. Oligomerizes upon binding of cytochrome c and dATP. Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains and consecutively mature caspase-9 is released from the complex. Pro-caspase-3 is recruited into the Apaf-1-pro-caspase-9 complex via interaction with pro-caspase-9. Interacts with APIP.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver.
DOMAIN: SwissProt: O14727 The CARD domain mediates interaction with APIP.
SIMILARITY: Contains 1 CARD domain. & Contains 1 NB-ARC domain. & Contains 13 WD repeats.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months. 包装信息 数量 100 µg
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