- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, B, M IHC, ELISA, WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB1632 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Nitric Oxide Synthase I Antibody Alternate Names - nNOS
- NOS I
- bNOS
产品信息 格式 Affinity Purified 演示 Peptide affinity-purified immunoglobulin. Supplied at a 1mg/mL solution in PBS, pH 7.4, containing 0.1% BSA. 应用 应用 Anti-Nitric Oxide Synthase I Antibody is an antibody against Nitric Oxide Synthase I for use in ELISA, WB, IH. 主要应用 - Immunohistochemistry
- ELISA
- Western Blotting
应用说明 Immunohistochemistry: Use at 5 - 10 μg/mL on rat neuronal sections fixed with 3.5% paraformaldehyde. Incubation overnight at 4°C with a 1:100 - 1:500 dilution. ABC (peroxidase) detection. Positive staining of Purkinje cells and Layer 5 primary neurons.
Western blot: 1 - 10 μg/mL with chemiluminescent detection. Higher concentrations of the antibody may be necessary if colorimetric detection is used. Antibody dilution will also vary with the concentration of antigen. Then antibody should recognize a 155 kDa protein.
ELISA: Use at 1.0 μg/mL or lower on plates coated with 50 - 100 ng of peptide antigen.
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Synthetic peptide corresponding to amino acids 724-739 of human brain NOS, coupled to KLH. 宿主 Rabbit 特异性 Reactive with human brain NOS-I (nNOS). Antibody binding is neutralized in Western blot by pre-adsorption with the immunogen peptide. This sequence is highly conserved in human, rat and mouse. 品种反应性 RatBovineMouse 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_000620.1
基因符号 - EC 1.14.13.39
- NOS1
- nNOS
- NC-NOS
- N-NOS
- IHPS1
- bNOS
- NOS
UniProt编号 - P29475
UniProt汇总 FUNCTION: SwissProt: P29475 # Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter.
COFACTOR: Heme group. & Binds 1 FAD. & Binds 1 FMN. & Metrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.
SIZE: 1434 amino acids; 160970 Da
SUBUNIT: Homodimer. Interacts with DLG4; the interaction possibly being prevented by the association between NOS1 and CAPON. Forms a ternary complex with CAPON and RASD1. Forms a ternary complex with CAPON and SYN1. Interacts with ZDHHC23. Interacts with NOSIP; which may impair its synaptic location (By similarity).
SUBCELLULAR LOCATION: Cell membrane, sarcolemma; Peripheral membrane protein. Cell projection, dendritic spine (By similarity). Note=In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines (By similarity).
TISSUE SPECIFICITY: Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland.
DOMAIN: SwissProt: P29475 The PDZ domain in the N-terminal part of the neuronal isoform participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes in muscles (By similarity).
DISEASE: SwissProt: P29475 # Genetic variations in NOS1 gene are associated with susceptibility to infantile hypertrophic pyloric stenosis type 1 (IHPS1) [MIM:179010]. IHPS has an incidence of 1-5 per 1'000 live births in whites and a marked preponderance of males to females (4:1). IHPS is the most frequent disorder requiring surgery in the first year of life. The disorder is characterized by hypertrophy and hyperplasia of the circular muscle layer of the pylorus, leading to persistent vomiting 2-12 weeks after birth. Defective pyloric relaxation and increased pyloric smooth muscle mass have been suggested to be responsible for gastric-outlet obstruction.
SIMILARITY: Belongs to the NOS family. & Contains 1 flavodoxin-like domain. & Contains 1 PDZ (DHR) domain.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated thawing and refreezing. 包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,14-739M,TLK2 Protein, active, 250 µg
下一件merck millipore产品：merck millipore,默克密理博,14-600,TAK1-TAB1 fusion Protein, active, 10 µg
相关产品
merck millipore,默克密理博,14-813,UbcH7 Conjugating Enzyme, 100 µg
merck millipore,默克密理博,MABN649,Anti-Potassium Channel KChIP1 Antibody
merck millipore,默克密理博,05-362,Anti-Cyclin D1/2 Antibody, clone 5D4
merck millipore,默克密理博,FCMAB206F,Milli-Mark™ Anti-CD69- FITC Antibody, clone FN50
merck millipore,默克密理博,14-739M,TLK2 Protein, active, 250 µg
merck millipore,默克密理博,14-600,TAK1-TAB1 fusion Protein, active, 10 µg
merck millipore,默克密理博,ABF117,Anti-IFIT1/p56 Antibody
merck millipore,默克密理博,01-106,FGF-2 / basic FGF Protein, Human recombinant
merck millipore,默克密理博,3455,D-Galactose - CAS 59-23-4 - Calbiochem
merck millipore,默克密理博,52332,Phenylmethylsulfonyl Fluoride - CAS 329-98-6 - Calbiochem