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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, M WB, IHC Rb Purified Polyclonal Antibody 描述 产品目录编号 AB1557P 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-NMDAR2B Antibody Alternate Names - glutamate receptor, ionotropic, N-methyl D-aspartate 2B
- Glutamate
- N-methyl D-aspartate receptor subtype 2B
- N-methyl-D-aspartate receptor subunit 2B
- N-methyl-D-aspartate receptor subunit 3
- glutamate receptor subunit epsilon-2
- ionotropic
- N-methyl D-aspartate 2B
背景信息 NMDA receptors are a class of ionotropic glutamate receptors. NMDA receptor channel has been shown to be involved in long term potentiation, an activity dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of the key receptor subunit NMDAR1 (GRIN1) and 1 or more of the 4 NMDAR2 subunits: NMDAR2A (GRIN2A), NMDAR2B (GRIN2B), NMDAR2C (GRIN2C), and NMDAR2D (GRIN2D). GRIN2B may be a candidate gene for the neurodegenerative disorder dentato-rubro-pallidoluysian atrophy (DRPLA).
Properties of NMDAR include modulation by glycine, inhibition by Zn2+, voltage dependent Mg2+ blockade and high Ca2+ permeability. The involvement of NMDAR in the CNS has become a focus area for neurodegenerative diseases such as Alzheimer's disease and also epilepsy and ischemic neuronal cell death.产品信息 格式 Purified 控制 - Brain tissue, Rat Purkinje cell dendrites, rat hippocampus lysate, HEK 293 cells expressing NR2B.
演示 Purified rabbit polyclonal lyophilized from ammonium bicarbonate (5 mM). Some residual salt may also be present. Reconstitute with 50 μL of PBS. Contains no preservatives. 应用 应用 Anti-NMDAR2B Antibody is an antibody against NMDAR2B for use in IH, IP & WB. 主要应用 - Western Blotting
- Immunohistochemistry
应用说明 Immunohistochemistry:
1:1,000-1:2,000 dilution of a previous lot was used on immunohistochemistry.
Immunoprecipitation:
3 μL of a previous lot of this antibody (under appropriate conditions) quantitatively immunoprecipitated all NMDAR2B in 200 μg of rat brain.
Western Blot Analysis:
1:100-1:2,000 dilution of a previous lot (NMDAR2B is present in high concentrations in the hippocampus).
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 C-Terminal Fusion Protein of the NMDAR2B (30 kDa). 表位 C-terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 NMDAR2B. Strong and specific immunolabeling of the 180 kDa NMDAR2B subunit of the NMDA receptor. No reactivity to NMDAR2A or NMDAR2C by Western blot. Immunolabeling has been blocked by preadsorption of the antibody with the C-terminal fusion protein that was used to generate the antibody. 品种反应性 RatHumanMouse 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_000834.2
Entrez基因汇总 N-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate receptors. NMDA receptor channel has been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of three different subunits: NR1 (GRIN1), NR2 (GRIN2A, GRIN2B, GRIN2C, or GRIN2D) and NR3 (GRIN3A or GRIN3B). The NR2 subunit acts as the agonist binding site for glutamate. This receptor is the predominant excitatory neurotransmitter receptor in the mammalian brain. 基因符号 - hNR3
- GRIN2B
- MGC142180
- NMDAR2B
- NR3
- NR2B
- MGC142178
纯化方法 Immunoaffinity purified UniProt编号 - Q13224
UniProt汇总 FUNCTION: SwissProt: Q13224 # NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.
SIZE: 1484 amino acids; 166367 Da
SUBUNIT: Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B). Found in a complex with GRIN1 and GRIN3B. Found in a complex with GRIN1, GRIN3A and PPP2CB. Interacts with PDZ domains of INADL and DLG4 (By similarity).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia.
SIMILARITY: SwissProt: Q13224 ## Belongs to the glutamate-gated ion channel (TC 1.A.10) family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20ºC from date of receipt. 包装信息 数量 10 µg
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