- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
-
-
merck millipore,默克密理博,AB1033,Anti-Versican Antibody, a.a. 1360-1439 of mouse versican
产品名称：Anti-Versican Antibody, a.a. 1360-1439 of mouse versican
产品型号：AB1033
Anti-Versican Antibody, a.a. 1360-1439 of mouse versican is an antibody against Versican for use in IP, WB, IH(P).
merck millipore,默克密理博,AB1033,Anti-Versican Antibody, a.a. 1360-1439 of mouse versican
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M WB Rb Purified Polyclonal Antibody 描述 产品目录编号 AB1033 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Versican Antibody, a.a. 1360-1439 of mouse versican Alternate Names - GAG beta Domain
背景信息 Versican is a large multi-domain chondroitin sulphate proteoglycan, is secreted by fibroblasts and other types of cells. It has a N-terminal hyaluronic acid (HA) binding domain. This domain has homology with the G1 and G2 domains of aggrecan, (G1 is the hyaluronic acid binding region), to cartilage link protein which stabilizes the binding of aggrecan to hyaluronic acid, and, to the hyaluronate receptor CD44. In addition, versican also has a lectin like domain, a C-terminal compliment repeat, and two EGF-like repeats, all of which can be found in aggrecan., Versican has an apparent molecular mass of ca. 1000 kDa, of which the core contributes 400 kDa from the 2409 amino acids, and the 12 -15 covalently attached chondroitin sulphate chains make up the rest. 产品信息 格式 Purified 控制 - Immunohistochemistry Positive control: surrounding cartilageWestern blotting positive control: total 13 day mouse embryo
演示 The immunoglobulin was affinity purified against the recombinant antigen and is presented in PBS. Anti-GST reactivity has not been adsorbed. Liquid in PBS. 应用 应用 Anti-Versican Antibody, a.a. 1360-1439 of mouse versican is an antibody against Versican for use in IP, WB, IH(P). 主要应用 - Western Blotting
应用说明 Immunohistochemistry (formally fixed, paraffin embedded): 10 μg/mL. Tissues must be treated with Chondroitinase before staining. {0.5U/mL in 100mM Tris-HCL pH 7.2-7.4, 30 minutes at room temperature} Then block and stain.
Western blotting: 0.5 μg/mL
Immunoprecipitation:
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 GST fusion protein containing amino acids 1360-1439 of mouse versican. 表位 a.a. 1360-1439 of mouse versican 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Mouse versican GAG beta domain. 品种反应性 Mouse 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_013227.2
- NM_001135.2
Entrez基因汇总 This gene is a member of the aggrecan/versican proteoglycan family. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Multiple alternatively spliced transcript variants that encode different protein isoforms have been observed in this gene. 基因符号 - CSPGCP
- ACAN
- MSK16
- SEDK
- CSPCP
- AGCAN
- AGC1
- CSPG1
纯化方法 Immunoaffinity purified UniProt编号 - P16112
UniProt汇总 FUNCTION: SwissProt: P16112 # This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.
SIZE: 2415 amino acids; 250193 Da
SUBUNIT: Interacts with FBLN1 (By similarity).
SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix (By similarity).
TISSUE SPECIFICITY: Restricted to cartilages.
DEVELOPMENTAL STAGE: Expression was detected in chondrocytes throughout the developing skeleton.
DOMAIN: SwissProt: P16112 Two globular domains, G1 and G2, comprise the N-terminus of the proteoglycan, while another globular region, G3, makes up the C-terminus. G1 contains Link domains and thus consists of three disulfide-bonded loop structures designated as the A, B, B' motifs. G2 is similar to G1. The keratan sulfate (KS) and the chondroitin sulfate (CS) attachment domains lie between G2 and G3.
PTM: Contains mostly chondroitin sulfate, but also keratan sulfate chains, N-linked and O-linked oligosaccharides. The release of aggrecan fragments from articular cartilage into the synovial fluid at all stages of human osteoarthritis is the result of cleavage by aggrecanase.
DISEASE: SwissProt: P16112 # Defects in AGC1 are the cause of spondyloepiphyseal dysplasia type Kimberley (SEDK) [MIM:608361]. Spondyloepiphyseal dysplasias are a heterogeneous group of congenital chondrodysplasias that specifically affect epiphyses and vertebrae. The autosomal dominant SEDK is associated with premature degenerative arthropathy.
SIMILARITY: Belongs to the aggrecan/versican proteoglycan family. & Contains 1 C-type lectin domain. & Contains 1 EGF-like domain. & Contains 1 Ig-like V-type (immunoglobulin-like) domain. & Contains 4 Link domains. & Contains 1 Sushi (CCP/SCR) domain.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeat freeze/thaw cycles. 包装信息 数量 50 µg
上一件merck millipore产品：merck millipore,默克密理博,03-101,RIPAb+ PABPC1 - RIP Validated Antibody and Primer Set
下一件merck millipore产品：merck millipore,默克密理博,04-1131,Anti-Integrin alpha4 Antibody, clone EPR1355Y, rabbit monoclonal
相关产品
merck millipore,默克密理博,AB5403,Anti-NMDAR2B Antibody, phosphoTyr 1472
merck millipore,默克密理博,164610,p-Aminophenylmercuric Acetate - CAS 6283-24-5 - Calbiochem
merck millipore,默克密理博,482650,Nitric Oxide Assay Kit, Colorimetric
merck millipore,默克密理博,16-224,Anti-Myc Tag Antibody, clone 4A6, Alexa Fluor 488 conjugate
merck millipore,默克密理博,03-101,RIPAb+ PABPC1 - RIP Validated Antibody and Primer Set
merck millipore,默克密理博,04-1131,Anti-Integrin alpha4 Antibody, clone EPR1355Y, rabbit monoclonal
merck millipore,默克密理博,6701,Uridine-5ʹ-triphosphate, Trisodium Salt - CAS 19817-92-6 - Calbiochem
merck millipore,默克密理博,MABN50A4,Anti-Olig2 Antibody, clone 211F1.1, Alexa Fluor488 Conjugate,MABN50A4
merck millipore,默克密理博,14-734M,FGFR1 (V561M) Protein, active, 250 µg
merck millipore,默克密理博,MABS477,Anti-LAP (TGF-β1) Antibody, clone TW4-6H10