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检测方法 Colorimetric 描述 概述 Assay kit for the rapid quantitative measurement of total nitric oxide (NO). Based on the enzymatic conversion of nitrate to nitrite by nitrate reductase, followed by the spectrophotometric quantitation of nitrite levels using Griess Reagent. Do not use with nitrate- or nitrite-containing tissue culture media such as RPMI. 产品目录编号 482650 品牌系列 Calbiochem®
参考 参考 Nims, R.W., et al. 1996. Methods Enzymol. 268, 93. 产品信息 检测方法 Colorimetric 形式 80 Tests 格式 96-well plate 试剂盒包含 Recombinant Zea mays Nitrate Reductase, NADH, Nitrate Standard, Color Reagents, Buffer, 96-Well Plate, and a user protocol. 生物信息 化验时间 1-1.5 h 样品类型 Aqueous solutions 安全信息 R相 R: 34-36/37/38-40-42/43-20/21/22
Causes burns.
Irritating to eyes, respiratory system and skin.
Limited evidence of a carcinogenic effect.
May cause sensitization by inhalation and skin contact.
Harmful by inhalation, in contact with skin and if swallowed.S相 S: 26-36-45
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).产品使用声明 预定用途 This Calbiochem® Nitric Oxide Assay Kit, Colorimetric is intended for the spectrophotometric measurement of the nitric oxide (NO) produced in in vitro experimental systems. This method can be used to accurately measure as little as 1 µM (pmol/ml) NO in aqueous solutions and requires small quantities of samples (5 to 85 µl, depending on the concentration of nitric oxide in the sample). 储存和货运信息 货运代码 Blue Ice Only
毒性 Multiple Toxicity Values, refer to MSDS
储存 +2°C to +8°C
存储条件 Upon arrival immediately store the Nitrate Reductase at -20°C immediately and all other components at 4°C. 避光 Protect from light
无需冷冻 No
补充信息 试剂盒包含 Recombinant Zea mays Nitrate Reductase, NADH, Nitrate Standard, Color Reagents, Buffer, 96-Well Plate, and a user protocol.
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