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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Ch, Av, H, M, R WB, IHC M Purified Monoclonal Antibody 描述 产品目录编号 05-172 品牌系列 Upstate
商名 - Upstate
描述 Anti-IGF-I Antibody, clone Sm1.2 Alternate Names - somatomedin C
- Mechano growth factor
- insulin-like growth factor 1
- insulin-like growth factor 1 (somatomedin C)
背景信息 Insulin-like growth factor 1 (IGF-1) is a polypeptide protein hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. IGF-1 consists of 70 amino acids in a single chain with three intramolecular disulfide bridges.
IGF-1 is produced primarily by the liver as an endocrine hormone and targets tissues in a paracrine/autocrine fashion. Production is stimulated by growth hormone and can be retarded by undernutrition, growth hormone insensitivity, lack of growth hormone receptors, or failures of the downstream signaling pathway post GH receptor including SHP2 and STAT5b. Approximately 98% of IGF-1 is always bound to one of 6 binding proteins (IGF-BP). IGFBP-3, the most abundant protein, accounts for 80% of all IGF binding. IGF-1 binds to IGFBP-3 in a 1:1 molar ratio. Its primary action is mediated by binding to specific IGF receptors present on many cell types in many tissues. The signal is transduced by intracellular events. IGF-1 is one of the most potent natural activators of the AKT signaling pathway, a stimulator of cell growth and multiplication and a potent inhibitor of programmed cell death.产品信息 格式 Purified 控制 - MCF-7 cell extracts
演示 Purified mouse IgG1κ lyophilized powder, lyophilized from 105 μL of 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.1 mM EDTA. 应用 应用 Anti-IGF-I Antibody, clone Sm1.2 is an antibody against IGF-I for use in IH, IP, NEUT & WB. 主要应用 - Western Blotting
- Immunohistochemistry
应用说明 Immunoprecipitation:
5 μg of a previous lot immunoprecipitated 100 ng of IGF-I.
Immunohistochemistry:
10 μg/mL of a previous lot was reported by an independent laboratory to detect IFG-I in formalin-fixed, paraffin-embedded skin sections.
(Lui, 1994).
Neutralization:
10-20 μg/mL of a previous lot inhibited the activity of 10 ng/mL of IGF-I as determined by testing with chicken embryo fibroblasts (CEF) using an ATP endpoint assay (ATPLiteTM-M, Packard Instruments).生物信息 免疫原品种 Human insulin-like growth factor I (IGF-I) purified from human plasma. (van Wyk, J.J., 1986). 克隆 Sm1.2 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 IGF-I. (Russel, W.E., 1984). 40% cross reactivity to IGF-II. (van Wyk, J.J., 1997). 同种型 IgG1κ 品种反应性 ChickenAvianHumanMouseRat Species Reactivity Note Human, mouse, rat and possibly chicken.
Occasional difficulty neutralizing rat IGF-I due to binding protein interference.抗体类型 Monoclonal Antibody Entrez基因编号 - NP_000609
Entrez基因汇总 The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Several transcript variants encoding different isoforms have been found for this gene. 基因符号 - IGF-IB
- IGF1
- IBP1
- IGFI
- IGF-IA
- MGF
纯化方法 Protein G purfied UniProt编号 - P05019
UniProt汇总 FUNCTION: SwissProt: P05019 # The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity.| P01343 # The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity.
SIZE: 195 amino acids; 21841 Da
SUBCELLULAR LOCATION: Secreted.
DOMAIN: SwissProt: P05019
DISEASE: SwissProt: P05019 # Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation.| P01343 # Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation.
SIMILARITY: SwissProt: P05019 ## Belongs to the insulin family.| P01343 ## Belongs to the insulin family.产品使用声明 质量保证 Evaluated on IGF-I (Catalog # 01-208) under non-reducing conditions.
Western Blot Analysis:
0.5-2 μg/mL of this antibody detected 100 ng of IGF-I (Catalog # 01-208) under non-reducing conditions.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20ºC from date of receipt. 包装信息 数量 200 µg
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