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描述 概述 The BacMagic™-2 Transfection Kit provides a positive selection for creating baculovirus recombinants in preparation for recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification. Like BacMagic™ DNA, BacMagic-2 DNA is derived from an AcNPV genome, contains an ORF 1629 deletion which prevents nonrecombinant virus from replicating in insect cells, and contains a deletion of the nonessential chitinase (chiA) gene, to improve production of secreted and membrane-targeted proteins. BacMagic-2 DNA have an additional deletion of the v-cath gene, a cathespsin-like cysteine protease, resulting in significantly improved quality and yield for most target proteins. The BacMagic-2 Transfection Kit contains the BacMagic-2 DNA, Insect GeneJuice® Transfection Reagent, and a positive control plasmid to monitor co-transfection efficiency. In addition, it contains Sf9 cells and BacVector® Medium, for optimal transfection performance.
Not available for sale in Japan产品目录编号 72158 品牌系列 Novagen®
应用数据 
Figure 1. Construction of baculovirus recombinants with three BacMagic systems
Figure 2. Streamlined baculovirus method产品信息 部件 声明 Not available for sale in Japan. 安全信息 R相 R: 36/38
Irritating to eyes and skin.S相 S: 24/25-26-36/37/39
Avoid contact with skin and eyes.
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing, gloves and eye/face protection.储存和货运信息 货运代码 Multiple Storage Conditions
毒性 Multiple Toxicity Values, refer to MSDS
储存 Multiple storage requirements
无需冷冻 No
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