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描述 概述 The BacMagic™-1 DNA Kit provides a positive selection for creating baculovirus recombinants in preparation for generating recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification. BacMagic DNA is an AcNPV genome with a portion of the essential open reading frame (ORF) 1629 deleted, preventing nonrecombinant virus from replicating in insect cells. The target coding sequence in a compatible transfer plasmid, such as pIEx/Bac™, pBAC™, or pTriEx™ vectors, is cotransfected with BacMagic DNA into insect cells. Homologous recombination within the cells restores the function of the viral ORF1629. Only recombinant baculovirus can replicate, producing a homogeneous population of recombinants. The BacMagic™-1 DNA Kit contains the BacMagic-1 DNA, Insect GeneJuice® Transfection Reagent, and a positive control plasmid to monitor co-transfection efficiency.
Not available for sale in Japan产品目录编号 71545 品牌系列 Novagen®
应用数据 
Figure 1. Construction of baculovirus recombinants with three BacMagic systems
Figure 2: Streamlined baculovirus method产品信息 集合编号 TRANSFECTION CONTROL PLASMID 部件 储存和货运信息 货运代码 Blue Ice Only
毒性 Multiple Toxicity Values, refer to MSDS
储存 +2°C to +8°C
无需冷冻 No
上一件merck millipore产品：merck millipore,默克密理博,567549,Sodium Phosphate, Monobasic, Monohydrate, Molecular Biology Grade - CAS 10049-21-5 - Calbiochem
下一件merck millipore产品：merck millipore,默克密理博,428017,Anti-Lamp1 Mouse mAb (LY1C6)
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