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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M, R WB Rb Purified Polyclonal Antibody 描述 产品目录编号 07-225 品牌系列 Upstate
商名 - Upstate
描述 Anti-TrkB Antibody Alternate Names - tyrosine kinase receptor B
- BDNF/NT-3 growth factors receptor
- Neurotrophic tyrosine kinase receptor type 2
- TrkB tyrosine kinase
- neurotrophic tyrosine kinase, receptor, type 2
- receptor
- type 2
背景信息 The Trk (tropomyosin-receptor-kinase) proto-oncogene family of receptor tyrosine kinases contains four members, TrkA, TrkB, TrkC, and TrkE, which are variably expressed throughout the central and peripheral nervous systems. TrkB is the high-affinity to the binding brain-derived neurotrophic factor (BDNF) receptor and neurotrophin-4/5 NT-4/5). TrkB is autophosphorylated on five cytoplasmic tyrosines. The binding of BDNF to TrkB receptor causes many intercellular cascades to be activated such as promoting survival of sensory neurons mainly through Shc site-independent pathways.The signaling activity of these receptors includes the Ras – MAP kinase pathway, and the PI3 Kinase pathway. These receptors are responsible for resistance to apoptosis, obesity, Alzheimer’s disease, and mood disorders. 产品信息 格式 Purified 控制 - Brain tissue.
演示 Purified rabbit polyclonal in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide, and 30% glycerol. 应用 应用 This Anti-TrkB Antibody is validated for use in WB for the detection of TrkB. 主要应用 - Western Blotting
生物信息 免疫原品种 The entire extracellular domain (corresponding to residues 1–429) of the rat TrkB receptor, expressed in COS cells. The immunizing sequence has 97% identity with mouse TrkB and 88% identity with human TrkB. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recognizes TrkB, Mr 145 kDa, as well as an additional protein, Mr 90 kDa, which is likely a TrkB degradation product. 同种型 IgG 品种反应性 MouseRat Species Reactivity Note Rat and mouse. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_036863
Entrez基因汇总 Receptor for brain-derived neurotrophic factor (BDNF) [RGD]. 基因符号 - Trk-B
- NTRK2
- GP145-TrkB
- TRKB
纯化方法 Protein A purfied UniProt编号 - Q16620
UniProt汇总 FUNCTION: SwissProt: Q16620 # Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC- gamma-1.
SIZE: 822 amino acids; 91999 Da
SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. Binds SH2B2. Interacts with SQSTM1 (By similarity).
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Isoform TrkB is widely expressed, mainly in the nervous tissue. In the CNS, expression is observed in the cerebral cortex, hippocampus, thalamus, choroid plexus, granular layer of the cerebellum, brain stem, and spinal cord. In the peripheral nervous system, it is expressed in many cranial ganglia, the ophtalmic nerve, the vestibular system, multiple facial structures, the submaxillary glands, and dorsal root ganglia. Isoform TrkB-T1 is expressed in multiple tissues, mainly in brain, pancreas, kidney and heart. Isoform TrkB-T-Shc is predominantly expressed in brain.
PTM: Ligand-mediated auto-phosphorylation.
SIMILARITY: SwissProt: Q16620 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. & Contains 2 Ig-like C2-type (immunoglobulin-like) domains. & Contains 2 LRR (leucine-rich) repeats. & Contains 1 protein kinase domain.产品使用声明 质量保证 Evaluated by western blot on mouse brain membrane protein preparations.
Western Blot Analysis:
0.5-2 µg/mL of this antibody detected TrkB in mouse brain membrane protein preparations.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20ºC from date of receipt. 包装信息 数量 200 µg
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