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品种反应性 主要应用 宿主 格式 抗体类型 R, H WB, ICC Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB5471P Replaces AB5471 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Semaphorin 3F Antibody 产品信息 格式 Affinity Purified 控制 - Embryonic brain tissue, neural growth cones
演示 Affinity Purified immunoglobulin. Liquid in 0.02M Phosphate buffer with 0.25M NaCl and 0.1% sodium azide. 应用 应用 Anti-Semaphorin 3F Antibody is an antibody against Semaphorin 3F for use in IC & WB. 主要应用 - Western Blotting
- Immunocytochemistry
应用说明 Western blot: 0.5-1.0 μg/mL using ECL. Suggested antibody dilution buffer is TBST (10 mM Tris-HCl, pH 8.0, 150 mM NaCl with 0.05% Tween 20). Suggested incubation is for two hours at room temperature or overnight at 2-8°C.
Immunocytochemistry.
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Synthetic peptide from human Semaphorin 3F (Catalog number AG363). (TPWTQTQAVRFRFSRATDGALRPM). 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recognizes Semaphorin 3F (Sema3F), recombinant and purified protein. By Western blot the antibody recognizes a protein of 90 kDa in total extracts from tissue culture cells producing Sema3F. Semaphorin 3F is expressed ubiquitously with higher levels in lung, brain, spleen, muscle and heart. 品种反应性 RatHuman Species Reactivity Note This antibody may also react with mouse because of extremely high sequence conservation in the SEM3F protein. 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_004186.2
Entrez基因汇总 The semaphorins are a family of proteins that are involved in signaling. All the family members have a secretion signal, a 500-amino acid sema domain, and 16 conserved cysteine residues (Kolodkin et al., 1993 [PubMed 8269517]). Sequence comparisons have grouped the secreted semaphorins into 3 general classes, all of which also have an immunoglobulin domain. The semaphorin III family, consisting of human semaphorin III (SEMA3A; MIM 603961), chicken collapsin, and mouse semaphorins A, D, and E, all have a basic domain at the C terminus. Chicken collapsin contributes to path finding by axons during development by inhibiting extension of growth cones (Luo et al., 1993 [PubMed 8402908]) through an interaction with a collapsin response mediator protein of relative molecular mass 62K (CRMP62) (Goshima et al., 1995 [PubMed 7637782]), a putative homolog of an axonal guidance associated UNC33 gene product (MIM 601168). SEMA3F is a secreted member of the semaphorin III family.[supplied by OMIM] 基因符号 - sema4
- SEMA3F
- SEMAK
- SEMA-IV
- SEMA4
- Sema4
纯化方法 Immunoaffinity purified UniProt编号 - Q13275
UniProt汇总 FUNCTION: SwissProt: Q13275 # May play a role in cell motility and cell adhesion.
SIZE: 785 amino acids; 88381 Da
SUBCELLULAR LOCATION: Secreted (By similarity).
TISSUE SPECIFICITY: Expressed abundantly but differentially in a variety of neural and nonneural tissues. There is high expression in mammary gland, kidney, fetal brain, and lung and lower expression in heart and liver.
DEVELOPMENTAL STAGE: Detected as early as embryonic day 10.
SIMILARITY: SwissProt: Q13275 ## Belongs to the semaphorin family. & Contains 1 Ig-like C2-type (immunoglobulin-like) domain. & Contains 1 Sema domain.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 包装信息 数量 100 µg
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