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描述 概述 The pIEx™ vectors are designed for cloning and high-level expression of proteins in transiently transfected Spodoptera-derived insect cells. Transcription is driven by the AcNPV-derived hr5 enhancer and immediate early promoter, IE1. pIEx-2 contains N-terminal GST•Tag, His•Tag, and S•Tag coding sequences, and a C-terminal HSV•Tag coding sequence. N-terminal vector encoded sequences can be completely removed by cloning into the PshA I site and cleaving the fusion protein with enterokinase.
pIEx-2 is also available as an Ek/LIC vector. The Ek/LIC vector is prepared for rapid directional cloning of PCR-amplified DNA. Using specifically designed primers for amplification, inserts can be efficiently cloned without the need for restriction enzyme digestion or ligation.
For sequencing or PCR of recombinants in pIEx-2, refer to the table below to choose the appropriate primers.Vector 5′ Primer 3′ Primer alt 5′ Primer pIEx-1 S•Tag 18mer Primer AS HSV•Tag Primer IE1 Promoter Primer pIEx-2 S•Tag 18mer Primer AS HSV•Tag Primer IE1 Promoter Primer pIEx-3 S•Tag 18mer Primer AS HSV•Tag Primer IE1 Promoter Primer pIEx-4 IE1 Promoter Primer AS S•Tag 18mer Primer NA pIEx-5 IE1 Promoter Primer AS S•Tag 18mer Primer NA pBiEx-1 S•Tag 18mer Primer AS HSV•Tag Primer IE1 Promoter Primer
T7 Promoter PrimerpBiEx-2 S•Tag 18mer Primer AS HSV•Tag Primer IE1 Promoter Primer
T7 Promoter PrimerpBiEx-3 IE1 Promoter Primer AS S•Tag 18mer Primer T7 Promoter Primer 产品目录编号 71238 品牌系列 Novagen®
同义词 InsectDirect™ System- pIEx™-2 DNA 储存和货运信息 货运代码 Shipped with Blue Ice or with Dry Ice
毒性 Standard Handling
储存 -20°C
无需冷冻 No
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