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品种反应性 主要应用 宿主 格式 抗体类型 R, H, M WB Rb Purified Polyclonal Antibody 描述 产品目录编号 AB3613 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Caspase 12 Antibody, NT 背景信息 Three distinct signaling pathways lead to apoptosis. The death receptor and mitochondrion pathways are the two mains, which involve respectively caspase-8 and caspase-9. The endoplasmic reticulum stress is the third apoptotic pathway and involves caspase-12. Localized to the ER but not to cytoplasm or mitochondria, caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis. Caspase-12 is co-localized to the ER with several proteins that are involved in Alzheimer's disease including g-secretase presenilin and b-amyloid precursor protein (APP). Caspase-12 mediates ER stress-induced apoptosis as well as cytotoxicity induced by amyloid-b. Caspase-12 is ubiquitously expressed in mouse tissues. 产品信息 格式 Purified 演示 Affinity purified immunoglobulin. Liquid in PBS containing 0.02% sodium azide. 应用 应用 This Anti-Caspase 12 Antibody, N-terminus is validated for use in WB for the detection of Caspase 12. 主要应用 - Western Blotting
应用说明 Western blot: 1:500 to 1:1000. Detects a band at ~53-49 kDa. in normal tissue extracts. Caspase 12 is a stress induced caspase localized to the ER and activated upon ER stress. Active process forms are difficult to detect in most non-transfected systems. Not all cells produce caspase 12; mouse L929 fibrosacrcoma cells are an excellent positive control line.
Mouse brain tissue lysate can be used as a positive control as well. Caspase 12 is associated with ER membranes and not in cytoplasm or mitochondria. Caspase 12 mediates cytotoxixity induced by amyloid beta.
Immunohistochemistry: 10μg/mL
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Peptide corresponding to aa 2-17 of mouse caspase-12. 表位 N-terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Antibody recognizes caspase 12 pro-protein in mouse extracts of normal and apoptotic cells. Caspase 12 is predicted to have a large >120aa long prodomain region. Thus this antibody recognizes only the proforms of the enzyme and not the active subunits. Uncleaved (proform) caspase 12 is prominently detected in many tissues. Active forms are difficult to detect expect in transfect cell systems. {Kalai, M et al (2003) 162:457-467}. 品种反应性 RatHumanMouse 抗体类型 Polyclonal Antibody Entrez基因汇总 Caspases are cysteine proteases that cleave C-terminal aspartic acid residues on their substrate molecules. This gene, CASP12, is most highly related to members of the ICE subfamily of caspases that process inflammatory cytokines. In rodents, the homolog of this gene mediates apoptosis in response to endoplasmic reticulum stress. However, in humans this gene contains a polymorphism for the presence or absence of a premature stop codon. The majority of human individuals have the premature stop codon and produce a truncated non-functional protein. The read-through codon occurs primarily in individuals of African descent and carriers have endotoxin hypo-responsiveness and an increased susceptibility to severe sepsis. 基因符号 - CASP12P1
- CASP12
- Caspase-12.
UniProt编号 - Q6UXS9
UniProt汇总 SIZE: 341 amino acids; 38907 Da 产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at 4°C for up to 6 months. 包装信息 数量 100 µg
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