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描述 概述 When used with Novagen's Streptavidin Alkaline Phosphatase Conjugate, Biotinylated T7•Tag Antibody enables a 5-fold increase in detection sensitivity of target proteins on Western blots compared to an anti-IgG conjugate strategy. This form of the antibody also enables specific detection in the presence of other mouse IgGs. The T7•Tag® Antibody is a mouse monoclonal antibody (IgG2b) directed against the 11 amino acid gene 10 leader peptide expressed by many of the pET translation vectors as well as pSCREEN™ and pRSET vectors. Because the peptide is the natural amino terminal end of the T7 major capsid protein, the antibody also recognizes T7 bacteriophage (e.g., the T7Select™ Vectors).
This antibody is also available as a direct conjugate with alkaline phosphatase or horseradish peroxidase.
Specificity T7•Tag peptide MetAlaSerMetThrGlyGlyGlnGlnMetGly; N-terminal, C-terminal or internal Species/Isotype Mouse monoclonal IgG2b Cross-reactivity Negligible with bacterial, insect or mammalian cell lysates Sensitivity 1–10 ng (Western blot developed with chromogenic substrates)
< 1 ng (developed with chemiluminescent substrates)Form Stabilized solution of antibody conjugate in 50% glycerol; includes Positive Control Extract containing 31.1 kDa target protein Working dilution 1:5,000–1:10,000 产品目录编号 69968 品牌系列 Novagen®
产品信息 部件 储存和货运信息 货运代码 Shipped with Blue Ice or with Dry Ice
毒性 Multiple Toxicity Values, refer to MSDS
储存 -20°C
避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
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