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描述 概述 A chemically modified trypsin that is resistant to autolysis and degradation. Trypsin specifically hydrolyzes amides, peptides and proteins at the C-terminus side of arginine and lysine residues. This mass spectrometry grade trypsin is methylated, TPCK treated, and affinity purified. It is specifically designed for digestion of proteins for sequencing and peptide fragment analysis by mass spectrometry. A trypsin to protein ratio of 1:100 to 1:20 is recommended for mass spectrometry protocols. Note: 1 SET = 5 X 20 µg of modified trypsin, and 1 ml of resuspension buffer. 产品目录编号 650279 品牌系列 Calbiochem®
参考 参考 Henzel, W.J., et al. 1993. PNAS 90, 5011.
Rice, R.H., et al. 1977. Biochim Biophys Acta 492, 316..产品信息 形式 Lyophilized Molar Mass 24000 生物信息 特异活性 ≥10,000 U/mg 理化信息 污染物 Chymostrypsin Activity: <3 BTEE U/mg (one BTEE unit is defined as the amount of enzyme required to hydrolyze 1 µmol BTEE per min at 25°C, pH 7.8.) 安全信息 R相 R: 36/37/38-42
Irritating to eyes, respiratory system and skin.
May cause sensitization by inhalation.S相 S: 22-24-26-36/37-45
Do not breathe dust.
Avoid contact with skin.
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing and gloves.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).储存和货运信息 货运代码 Blue Ice Only
毒性 Harmful
储存 -20°C
无需冷冻 No
特殊指南 Following reconstitution, aliquot and freeze (-°C). Unused stock solutions are stable for up to 2 weeks at -70°C. Avoid freeze/thaw cycles of solution.
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