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merck millipore,默克密理博,05-922R,Anti-phospho-Estrogen Receptor alpha (Ser305) Antibody, clone 124.9.4, rabbit monoclonal
产品名称：Anti-phospho-Estrogen Receptor alpha (Ser305) Antibody, clone 124.9.4, rabbit monoclonal
产品型号：05-922R
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.
merck millipore,默克密理博,05-922R,Anti-phospho-Estrogen Receptor alpha (Ser305) Antibody, clone 124.9.4, rabbit monoclonal
- 产品介绍
- merck millipore,默克密理博,05-922R,Anti-phospho-Estrogen Receptor alpha (Ser305) Antibody, clone 124.9.4, rabbit monoclonal
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H IHC, WB Rb Purified Monoclonal Antibody 描述 产品目录编号 05-922R 描述 Anti-phospho-Estrogen Receptor alpha (Ser305) Antibody, clone 124.9.4, rabbit monoclonal Alternate Names - Nuclear receptor subfamily 3 group A member 1
- Estrogen receptor
- ER-alpha
- Estradiol receptor
背景信息 Estrogen Receptor α (ERα) is a nuclear hormone receptor. This steroid hormone receptor is involved in the regulation of eukaryotic gene expression and affects cellular proliferation and differentiation in target tissues. ERα is a ligand-activated transcription factor and upon being bound by a ligand such as estrogen, a conformational change occurs that allows dimerization and binding to an estrogen response element. When bound to DNA, Estrogen Receptor α can positively or negatively regulate gene transcription. Like other steroid hormone receptors, Estrogen Receptors are intracellular proteins. Two ERs are known, ERα, classically known as ER, and ERβ. The Estrogen Receptor is believed to play an important role in the growth and development of a subset of hormone dependent human breast cancers. ERβ is expressed in prostate, ovary, and other reproductive organs. Although its steroid specificity is very similar to that of the ERα, the molecular mechanisms regulating the transcriptional activity of ERβ appears to be distinct from those regulating ERα. 产品信息 格式 Purified 控制 - PKA phosphorylated and unphosphorylated recombinant ERα protein.
演示 Purified rabbit monoclonal IgG in buffer containing PBS with 0.05% sodium azide. 应用 应用 Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. 主要应用 - Immunohistochemistry
- Western Blotting
应用说明 Immunohistochemistry Analysis: A 1:200 dilution from a representative lot detected Estrogen Receptor alpha in ductal carcinoma. 生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to human Estrogen Receptor alpha phosphorylated at Ser305. 表位 Phosphorylated Ser305 克隆 124.9.4 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Thia antibody recognizes Estrogen Receptor alpha phosphorylated at Ser305. 同种型 IgG 品种反应性 Human Species Reactivity Note Demonstrated to react with Human. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_000116
Entrez基因汇总 Summary: This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters. [provided by RefSeq]. 基因符号 - NR3A1
- ESR1
- ESR
纯化方法 Protein A purified UniProt编号 - P03372
UniProt汇总 FUNCTION: Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.
SUBUNIT STRUCTURE: Binds DNA as a homodimer. Can form a heterodimer with ESR2. Interacts with FOXC2, MAP1S, SLC30A9, UBE1C and NCOA3 coactivator (by similarity). Interacts with EP300; the interaction is estrogen-dependent and enhanced by CITED1. Interacts with CITED1; the interaction is estrogen-dependent. Interacts with NCOA5 and NCOA6 coactivators. Interacts with NCOA7; the interaction is a ligand-inducible. Interacts with PHB2, PELP1 and UBE1C. Interacts with AKAP13. Interacts with CUEDC2. Interacts with KDM5A. Interacts with SMARD1. Interacts with HEXIM1. Interacts with PBXIP1. Interaction with MUC1 is stimulated by 7 beta-estradiol (E2) and enhances ERS1-mediated transcription. Interacts with DNTTIP2, FAM120B and UIMC1. Interacts with isoform 4 of TXNRD1. Interacts with MLL2. Interacts with ATAD2 and this interaction is enhanced by estradiol. Interacts with KIF18A and LDB1. Interacts with RLIM (via C-terminus). Interacts with MACROD1. Interacts with SH2D4A and PLCG. Interaction with SH2D4A blocks binding to PLCG and inhibits estrogen-induced cell proliferation. Interacts with DYNLL1. Interacts with CCDC62 in the presence of estradiol/E2; this interaction seems to enhance the transcription of target genes. Interacts with NR2C1; the interaction prevents homodimerization of ESR1 and suppresses its transcriptional activity and cell growth. Interacts with DYX1C1. Interacts with PRMT2. Interacts with PI3KR1 or PI3KR2, SRC and PTK2. Interacts with RBFOX2.
SUBCELLULAR LOCATION: Nucleus. Cytoplasm
DOMAIN: Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
POST-TRANSLATIONAL MODIFICATION: Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity. Glycosylated; contains N-acetylglucosamine, probably O-linked. Ubiquitinated. Deubiquitinated by OTUB1. Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
POLYMORPHISM: Genetic variations in ESR1 are correlated with bone mineral density (BMD). Low BMD is a risk factor for osteoporotic fracture. Osteoporosis is characterized by reduced bone mineral density, disrutption of bone microarchitecture, and the alteration of the amount and variety of non-collagenous proteins in bone. Osteoporotic bones are more at risk of fracture.
SEQUENCE SIMILARITIES: Belongs to the nuclear hormone receptor family. NR3 subfamily. Contains 1 nuclear receptor DNA-binding domain.产品使用声明 质量保证 Evaluated by Western Blot in PKA phosphorylated and unphosphorylated recombinant ERα protein.
Western Blot Analysis: 1µg/mL dilution of this antibody detected Estrogen Receptor alpha on 10 µg of PKA phosphorylated and unphosphorylated recombinant ERα protein.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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