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品种反应性 主要应用 宿主 格式 抗体类型 M FC, WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 06-1328 描述 Anti-Themis Antibody Alternate Names - Thymocyte-expressed molecule involved in selection
- thymocyte selection associated
- thymocyte selection pathway associated
- chromosome 6 open reading frame 190
- chromosome 6 open reading frame 207
- protein THEMIS
背景信息 Thymocyte-expressed molecule involved in selection (Themis) is a highly conserved protein that has been shown to serve a key role in regulating T-cell development through TCR signaling, especially through the moderation of phosphorylation and calcium influx. It is also a critical component in the later stages of thymocyte development through the regulation of positive/negative T-cell selection, and is required for integration and maintenance of signals necessary to T-cell lineage commitment and maturation. Themis expression is observed in thymus, spleen, thymocytes between Pre-TCR and checkpoints positive in selectin, and at low levels in mature T-cells. 产品信息 格式 Affinity Purified 控制 - Mouse thymocyte tissue lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Detect Themis using this Anti-Themis Antibody validated for use in WB, FC. 主要应用 - Flow Cytometry
- Western Blotting
应用说明 Western Blot (Snap i.d.) Analysis: 2 µg/mL from a previous lot detected Themis in 10 µg of mouse thymocyte tissue lysate.
Flow Cytometry Analysis: A previous lot was used by an independent laboratory in FC. (Vasily Rybakin, PhD., Gascoigne lab, Scripps Research Institute, La Jolla, CA
Western Blot Analysis: A previous lot of this antibody was used to detect the presence and absence of Themis in Themis KO or WT thymocytes (Fu, G., et al. (2013) Nature. doi: 10.1038/nature12718)
Immunopreciptiation Analysis: A previous lot of this antibody was used to immunoprecipitate Themis in WT thymocytes but not in Themis KO thymocytes (Fu, G., et al. (2013) Nature. doi: 10.1038/nature12718)生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to the C-terminus of mouse Themis. 表位 C-terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes Themis at the C-terminus. 品种反应性 Mouse Species Reactivity Note Demonstrated to react with mouse.
Rat (73% sequence homology).抗体类型 Polyclonal Antibody Entrez基因编号 - NP_848781
基因符号 - TSEPA
- Themis
纯化方法 Affinity Purified UniProt编号 - Q8BGW0
UniProt汇总 FUNCTION: Plays a central role in late thymocyte development by controlling both positive and negative T-cell selection. Required to sustain and/or integrate signals required for proper lineage commitment and maturation of T-cells. Regulates T-cell development through T-cell antigen receptor (TCR) signaling and in particular through the regulation of calcium influx and phosphorylation of Erk.
SUBUNIT STRUCTURE: Interacts with PLCG1, ITK and GRB2.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus
TISSUE SPECIFICTY: Expressed in the thymus and to a lesser extent in the spleen but not detectable in nonlymphoid tissues. Highly expressed in thymocytes between the pre-T-cell antigen receptor (pre-TCR) and positive-selection checkpoints and expressed at low level in mature T-cells (at protein level).
INDUCTION: Down-regulated by stimulation through the alpha-beta TCR.
PTM: Phosphorylated on Tyr residues quickly after TCR stimulation.
DISRUPTION: phenotype Mice are viable, are born at the expected mendelian frequency and present no gross abnormalities. They however display a blockade in late T-cell development with defects in thymocyte selection. The number of transitional CD4+CD8(int) thymocytes as well as CD4+ or CD8+ single-positive thymocytes is lower. Thymocytes show defective positive selection, resulting in fewer mature thymocytes. Negative selection is also impaired. A greater percentage of T-cells have CD4+CD25+Foxp3+ regulatory and CD62L(lo)CD44(hi) memory phenotypes compared to wild-type T-cells.
SEQUENCE SIMILARITIES: Belongs to the themis family.产品使用声明 质量保证 Evaluated by Western Blot in mouse thymocyte tissue lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected Themis in 10 µg of mouse thymocyte tissue lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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