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描述 概述 Native, rabbit isopeptidase T. A member of the higher molecular weight group of ubiquitin C-terminal hydrolases (UCHs) involved in the hydrolysis of isopeptide linkages of polyUb chains. Ipase-T activity is regulated by ubiquitin levels. Plays a major role in ubiquitin recycling and in the regulation of 26S proteasome activity. Recommended reaction buffer: 25 mM HEPES, 10 mM DTT, 25°C, pH 7.5. Pre-incubate the enzyme for 15 minutes with 10 mM DTT to achieve maximum activity. 产品目录编号 419700 品牌系列 Calbiochem®
同义词 Ipase-T 参考 参考 Dang, L.C., et al. 1998. Biochemistry 37, 1868.
Melandri, F.D., et al. 1996. Biochemistry 35, 12893.
Stein, R.L., et al. 1995. Biochemistry 34, 12616.
Pickart, C.M., and Rose, I.A. 1986. J. Biol. Chem. 261, 10210.产品信息 形式 Liquid 配方 In 150 mM NaCl, 50 mM HEPES, 1 mM DTT, pH 7.6. Molar Mass 100000 生物信息 生物活性 Fully functional as measured using 5 nM Ipase-T and 1 µM Ub-AMC (Cat. No. 662075) 纯度 ≥95% by SDS-PAGE 储存和货运信息 货运代码 Dry Ice Only
毒性 Standard Handling
储存 ≤ -70°C
避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
特殊指南 Following initial thaw, aliquot and freeze (-70°C).
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