merck millipore,默克密理博,05-781R,Anti-p70 S6 Kinase Antibody, clone 20-10C-6, rabbit monoclonal

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merck millipore,默克密理博,05-781R,Anti-p70 S6 Kinase Antibody, clone 20-10C-6, rabbit monoclonal
产品名称：Anti-p70 S6 Kinase Antibody, clone 20-10C-6, rabbit monoclonal
产品型号：05-781R
This Anti-p70 S6 Kinase Antibody, clone 20-10C-6 is validated for use in WB, IP, IC for the detection of p70 S6 Kinase.

merck millipore,默克密理博,05-781R,Anti-p70 S6 Kinase Antibody, clone 20-10C-6, rabbit monoclonal

产品介绍

merck millipore,默克密理博,05-781R,Anti-p70 S6 Kinase Antibody, clone 20-10C-6, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	ICC, WB	Rb	Unconjugated	Monoclonal Antibody

描述
产品目录编号	05-781R
Replaces	04-391
描述	Anti-p70 S6 Kinase Antibody, clone 20-10C-6, rabbit monoclonal
Alternate Names	serine/threonine kinase 14 alpha 70 kDa ribosomal protein S6 kinase 1 Ribosomal protein S6 kinase I p70 S6 kinase alpha p70 S6 kinase, alpha 1 p70 S6 kinase, alpha 2 ribosomal protein S6 kinase, 70kD, polypeptide 1 ribosomal protein S6 kinase, 70kDa, polypeptide 1
背景信息	S6 Kinase is a Ser/Thr kinase which phosphorylates the 40S ribosomal protein S6, and several translation-regulatory factors. S6 kinase is activated by phosphorylation of multiple sites by mTOR. S6K is an important regulator of protein synthesis, along with MAP Kinase. In neurons, S6K is linked to the cytoskeleton by neurabin, which may play a role in its activation. Rsk3 (MAPKAP Kinase 1c) and Rsk4 are also known as the Ribosomal Protein S6 Kinase, 90 kDa, polypeptide 2 and 6, respectively.

产品信息
格式	Unconjugated
控制	NIH/3T3, Hek293, and PC12 cell lysates
演示	Purified rabbit IgG supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide with 40% Glycerol.

应用
应用	This Anti-p70 S6 Kinase Antibody, clone 20-10C-6 is validated for use in WB, IP, IC for the detection of p70 S6 Kinase.
主要应用	Immunocytochemistry Western Blotting
应用说明	Western Blot (SNAP ID) Analysis: A 1:250 dilution from a previous lot detected p70 S6 Kinase on NIH/3T3, Hek293, and PC12 cell lysates. Immunoprecipiitation Analysis: 1 µg from a previous lot immunoprecipitated p70 S6 Kinase from 100 µg of HEK293 RIPA lysate. Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected p70 S6 Kinase in HeLa cells.

生物信息
免疫原品种	KLH-conjugated linear peptide corresponding to human p70 S6 Kinase at and around the N-terminus.
表位	N-terminus
克隆	20-10C-6
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	This antibody recognizes p70 S6 Kinase at the N-terminus.
同种型	IgG
品种反应性	RatHumanMouse
Species Reactivity Note	Reacts with human, mouse, and rat.
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_003152.1
Entrez基因汇总	This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding this gene and overexpression of this kinase are seen in some breast cancer cell lines. Alternate translational start sites have been described and alternate transcriptional splice variants have been observed but have not been thoroughly characterized. [provided by RefSeq].
基因符号	PS6K RPS6KB1 p70-alpha p70S6k p70-S6K p70 S6K S6Kalpha P70S6K STK14A S6K1 p70(S6K)-alpha
纯化方法	Protein A purfied
UniProt编号	P23443
UniProt汇总	FUNCTION: Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation. CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein. ENZYME REGULATION: Activated by serine/threonine phosphorylation and protein kinase C, inactivated by type 2A phosphatase. Activated by mTORC1. SUBUNIT STRUCTURE: Interacts with PPP1R9A/neurabin-1 By similarity. Interacts with RPTOR. SUBCELLULAR LOCATION: Cytoplasm By similarity. Cell junction › synapse › synaptosome By similarity. TISSUE SPECIFICITY: Widely expressed. Ref.2 PTM: Phosphorylation at Ser-421 is regulated by mTORC1. SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 protein kinase domain.

产品使用声明
质量保证	Evaluated by Western Blot in NIH/3T3, Hek293, and PC12 cell lysates. Western Blot Analysis: A 1:1,000 dilution of this antibody detected p70 S6 Kinase on NIH/3T3, Hek293 and PC12 cell lysates.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.