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描述 概述 Native N-Glycosidase F from Elizabethkingia meningosepticum. Catalyzes the hydrolysis of asparagine-linked high mannose, as well as hybrid and complex oligosaccharides from glycoproteins. This enzyme will not cleave oligosaccharides containing α1,3-linked core fucose commonly found on plant glycoproteins. N-Glycosidase F will also fail to cleave if the asparagine to which the oligosaccharide is attached is not peptide-bonded to at least one amino acid residue at both the amino and carboxy termini. 产品目录编号 362185 品牌系列 Calbiochem®
同义词 Glycopeptidase F, PNGase F 参考 参考 Means, R.E. and Desrosiers, R.C. 2000. J. Virol. 74, 11181.
Fan, J.Q., and Lee, Y.C. 1997. J. Biol. Chem. 272, 27058.
Tarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol. 230, 44.
Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem. 266, 1646.产品信息 CAS号 83534-39-8 活性 ≥4500 units/ml EC号 3.5.1.52 形式 Liquid 配方 In 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, pH 7.5. Molar Mass 36000 生物信息 特异活性 ≥20,000 units/mg protein 理化信息 污染物 Proteases: none detected. 储存和货运信息 货运代码 Blue Ice Only
毒性 Standard Handling
储存 +2°C to +8°C
无需冷冻 Yes
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