merck millipore,默克密理博,17-662,ChIPAb+ EZH2, clone AC22

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merck millipore,默克密理博,17-662,ChIPAb+ EZH2, clone AC22 - ChIP Validated Antibody and Primer Set
产品名称：ChIPAb+ EZH2, clone AC22 - ChIP Validated Antibody and Primer Set
产品型号：17-662
EZH2, clone AC22, ChIP validated antibody & primer set including the ChIP-grade antibody & the specific control PCR primers used for chromatin immunoprecipitation of EZH2.

merck millipore,默克密理博,17-662,ChIPAb+ EZH2, clone AC22 - ChIP Validated Antibody and Primer Set

产品介绍

merck millipore,默克密理博,17-662,ChIPAb+ EZH2, clone AC22 - ChIP Validated Antibody and Primer Set

重要规格表

品种反应性	主要应用
R, H, M, Vrt	ChIP, WB

描述
产品目录编号	17-662
品牌系列	Upstate
商名	Upstate ChIPAb+
描述	ChIPAb+ EZH2, clone AC22 - ChIP Validated Antibody and Primer Set
概述	All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction. The ChIPAb+ EZH2, clone AC22 antibody/primer set includes the EZH2, clone AC22 antibody, a negative control antibody (purified mouse IgG), and qPCR primers which amplify a 135 bp region within the promoter of the human MYT-1 gene. The EZH2, clone AC22 antibody and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of EZH2-associated chromatin.
Alternate Names	ENX-1 Enhancer of zeste homolog 2 Lysine N-methyltransferase 6 enhancer of zeste 2 enhancer of zeste homolog 2
背景信息	EZH2 is a polycomb group (PcG) protein that is a catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4 and the nuclear receptor RORA. Regulates the circadian clock via histone methylation at the promoter of the circadian genes. Essential for the CRY1/2-mediated repression of the transcriptional activation of PER1/2 by the CLOCK-ARNTL/BMAL1 heterodimer; involved in the di and trimethylation of 'Lys-27' of histone H3 on PER1/2 promoters which is necessary for the CRY1/2 proteins to inhibit transcription.

产品信息
格式	Purified
控制	Includes negative control mouse IgG antibody and primers specific for of human MYT-1 promoter region.
演示	EZH2, clone AC22 (mouse monoclonal IgG2a). One vial containing 50 μg of protein G purified antibody in 50 μL PBS containing 0.05% sodium azide. Store at -20°C. Normal Mouse IgG. Two vials, each containing 25 μg purified mouse IgG in 25 μL storage buffer containing 0.1% sodium azide. Store at -20°C. ChIP Primers MYT-1 promoter Region. One vial containing 75 μL of 5 μM of each primer specific for the promoter region of human MYT-1. Store at -20°C. FOR: ACA AAG GCA GAT ACC CAA CG REV: GCA GTT TCA AAA AGC CAT CC

应用
应用	EZH2, clone AC22, ChIP validated antibody & primer set including the ChIP-grade antibody & the specific control PCR primers used for chromatin immunoprecipitation of EZH2.
主要应用	Chromatin Immunoprecipitation (ChIP) Western Blotting
应用说明	Chromatin Immunoprecipitation: Representative lot data. Sonicated chromatin prepared from HeLa S3 cells (4 X 10⁶ cell equivalents per IP) was subjected to chromatin immunoprecipitation using 1 µg of either a normal mouse IgG or Anti-EZH2, clone AC22 antibody and the Magna ChIP G Kit (Cat. # 17-611). Successful immunoprecipitation of EZH2-associated DNA fragments was verified by qPCR using GAPDH promoter (negative) and MYT-1 promoter (positive) primers (Please see figures). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated. Please refer to the EZ-Magna G ChIP™ (Cat. # 17-409) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details. Immunoprecipitation Analysis: Representative lot data. HeLa nuclear cell extract was Immunoprecipitated with either anti-EZH2, clone AC22 or mouse IgG and then resolved by electrophoresis, transferred to PVDF and probed with Cat. # 05-1319 anti-EZH2, clone BD43 (1:1000 dilution). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP (Cat. # AP124P) and a chemiluminescence detection system. (Please see figures). Lane 1: Hela nuclear extract immunoprecipitate with anti-EZH2, clone AC22. Lane 2: Hela nuclear extract immunoprecipitate with IgG.

生物信息
免疫原品种	EZH2, clone AC22 purified antibody is made against a GST fusion protein corresponding to amino acids 353-451 of human EZH2.
表位	a.a. 353-451
宿主	Mouse
特异性	Recognizes EZH2, MW: ~85-100 kDa.
同种型	IgG2a
品种反应性	RatHumanMouseVertebrates
Species Reactivity Note	Human, mouse, rat. Not tested in other species. The immunogen sequence is identical in a wide range of animal and plant species, so broad cross-reactivity is expected.
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_004447.2
Entrez基因汇总	This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Two transcript variants encoding distinct isoforms have been identified for this gene.
基因符号	KMT6 EZH2
UniProt编号	Q15910
UniProt汇总	FUNCTION: Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A. CATALYTIC ACTIVITY: S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N(6)-methyl-L-lysine. SUBUNIT STRUCTURE: Binds ATRX via the SET domain Probable. Component of the PRC2/EED-EZH2 complex, which includes EED, EZH2, SUZ12, RBBP4 and RBBP7 and possibly AEBP2. The minimum components required for methyltransferase activity of the PRC2/EED-EZH2 complex are EED, EZH2 and SUZ12. The PRC2 complex may also interact with DNMT1, DNMT3A, DNMT3B and PHF1 via the EZH2 subunit and with SIRT1 via the SUZ12 subunit. Interacts with HDAC1 and HDAC2. SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Expressed in many tissues. Overexpressed in numerous tumor types including carcinomas of the breast, colon, larynx, lymphoma and testis. DEVELOPMENTAL STAGE: Expression decreases during senescence of embryonic fibroblasts (HEFs). Expression peaks at the G1/S phase boundary. INDUCTION: Expression is induced by E2F1, E2F2 and E2F3. Expression is reduced in cells subject to numerous types of stress including UV-, IR- and bleomycin-induced DNA damage and by activation of TP53/p53. PTM: Phosphorylated by AKT1. Phosphorylation by AKT1 reduces methyltransferase activity. SEQUENCE SIMILARITIES: Belongs to the histone-lysine methyltransferase family. EZ subfamily. Contains 1 SET domain. CAUTION: Two variants of the PRC2 complex have been described, termed PRC3 and PRC4. Each of the three complexes may include a different complement of EED isoforms, although the precise sequences of the isoforms in each complex have not been determined. The PRC2 and PRC4 complexes may also methylate 'Lys-26' of histone H1 in addition to 'Lys-27' of histone H3 (Ref.15 and Ref.19), although other studies have demonstrated no methylation of 'Lys-26' of histone H1 by PRC2

产品使用声明
质量保证	Chromatin Immunoprecipitation: Sonicated chromatin prepared from HeLa S3 cells (4 X 10⁶ cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µg of either a normal mouse IgG or Anti-EZH2, clone AC22 antibody and the Magna ChIP™ G Kit (Cat. # 17-611). Successful immunoprecipitation of EZH2-associated DNA fragments was verified by qPCR using Control Primers (Please see figures). Please refer to the EZ-Magna ChIP™ G (Cat. # 17-409) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品使用声明

质量保证

Chromatin Immunoprecipitation:
Sonicated chromatin prepared from HeLa S3 cells (4 X 10⁶ cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µg of either a normal mouse IgG or Anti-EZH2, clone AC22 antibody and the Magna ChIP™ G Kit (Cat. # 17-611). Successful immunoprecipitation of EZH2-associated DNA fragments was verified by qPCR using Control Primers (Please see figures).
Please refer to the EZ-Magna ChIP™ G (Cat. # 17-409) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.