merck millipore,默克密理博,17-10238,ChIPAb+™ HDAC3 Antibody

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merck millipore,默克密理博,17-10238,ChIPAb+™ HDAC3 Antibody
产品名称：ChIPAb+™ HDAC3 Antibody
产品型号：17-10238
This ChIPAb+ HDAC3 -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.

merck millipore,默克密理博,17-10238,ChIPAb+™ HDAC3 Antibody

产品介绍

merck millipore,默克密理博,17-10238,ChIPAb+™ HDAC3 Antibody

重要规格表

品种反应性	主要应用
M, B, H, Ht	ChIP, WB

描述
产品目录编号	17-10238
商名	Upstate ChIPAb+
描述	ChIPAb+™ HDAC3 Antibody
概述	All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction. The ChIPAb+ HDAC3 set includes the HDAC3 antibody, a Normal Mouse IgG, and control primers which amplify a 88 bp region of ChIP Primers, p21. The HDAC3 and negative controls are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of HDAC3-associated chromatin.
背景信息	Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. HDAC3 belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. HDAC3 is regarded as a potential tumor suppressor gene.

产品信息
格式	Purified
控制	Includes normal mouse IgG and primers specific for human p21.
演示	Anti-HDAC3 (mouse monoclonal). One vial containing 100 µg of protein G purified monoclonal in buffer containing 0.02 M phosphate buffer (pH7.6), 0.25M NaCl, 0.1% sodium azide and 30% glycerol. Store at -20°C. Concentration: 1 mg/mL Normal Mouse IgG. One vial containing 125 µg of purified mouse IgG in 125 µL of storage buffer containing 0.1% sodium azide. Store at -20°C. ChIP Primers, p21. One vial containing 75 μL of each primer (5 μM) specific for a region of the human p21 (WAF1/CIP1/CDKN1A) promoter. Store at -20°C. FOR: CCC ACA GCA GAG GAG AAA GAA REV: CTG GAA ATC TCT GCC CAG ACA

应用
应用	This ChIPAb+ HDAC3 -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
主要应用	Chromatin Immunoprecipitation (ChIP) Western Blotting
应用说明	Chromatin Immunoprecipitation: Representative lot data. Sonicated chromatin prepared from untreated or UV treated (6 hrs, 50 joules/m2.) U2OS cells (3 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 4 µg of either Normal Mouse IgG, or 4 µg of Anti-HDAC3 and the Magna ChIP™ A/G Kit (Cat. # 17-10085). Successful immunoprecipitation of HDAC3 associated DNA fragments was verified by qPCR using ChIP Primers, p21 .(Figure 2). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated. Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details. Western Blot Analysis: Representative lot data. HeLa nuclear extract was probed with Anti-HDAC3 (1 µg/mL). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates HDAC3 (~49 kDa). An uncharacterized band appears at ~22 in some lysates. (Figure 3).

生物信息
免疫原品种	peptide (NEFYDGDHDNDKESDVEI) corresponding to amino acids 411-428 of human HDAC3
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Mouse
同种型	IgG2a
品种反应性	MouseBovineHumanHamster
Species Reactivity Note	Demonstrated to react with human, bovine, human, and hamster. Predicted to react with rat and chicken based on 100% sequence homology.
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_003883
Entrez基因汇总	Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene.
基因符号	RPD3-2 HDAC3 SMAP45 HD3 RPD3
UniProt编号	O15379
UniProt汇总	FUNCTION: SwissProt: O15379 # Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. SIZE: 428 amino acids; 48848 Da SUBUNIT: Interacts with HDAC7 and HDAC9. Forms a heterologous complex at least with YY1. Interacts with DAXX, HDAC10 and DACH1. Found in a complex with NCOR1 and NCOR2. Component of the N-Cor repressor complex, at least composed of NCOR1, NCOR2, HDAC3, TBL1X, TBL1R, CORO2A and GPS2. Interacts with BCOR, MJD2A/JHDM3A, NRIP1 and SRY. Interacts with BTBD14B. Interacts with GLIS2 (By similarity). Interacts with CBFA2T3. SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Widely expressed. PTM: Sumoylated in vitro. SIMILARITY: SwissProt: O15379 ## Belongs to the histone deacetylase family. Type 1 subfamily.

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.