merck millipore,默克密理愽
  • merck millipore,默克密理博,17-191,MAP Kinase/Erk Assay Kit, non-radioactive

  • merck millipore,默克密理博,17-191,MAP Kinase/Erk Assay Kit, non-radioactive

    产品名称：MAP Kinase/Erk Assay Kit, non-radioactive
    产品型号：17-191
    MAP Kinase/Erk Assay Kit is designed to measure the phosphotransferase activity of MAP (mitogen-activated protein) Kinase in immunoprecipitates & column fractions.

    merck millipore,默克密理博,17-191,MAP Kinase/Erk Assay Kit, non-radioactive

  • 产品介绍
  • merck millipore,默克密理博,17-191,MAP Kinase/Erk Assay Kit, non-radioactive

    重要规格表

    主要应用 可用的分析物
    KAMAP Kinase/Erk
    描述
    产品目录编号 17-191
    品牌系列 Upstate
    商名
    • Upstate
    描述 MAP Kinase/Erk Assay Kit, non-radioactive
    产品信息
    部件
    • Anti-phospho-MBP, clone P12 (Cat.# 05-429)
    • Assay Dilution Buffer I (ADBI) (Cat.# 20-108)
    • MAP Kinase/Erk Substrate Cocktail II (Cat.# 20-166)
    • Inhibitor Cocktail (Cat.# 20-116)
    • Magnesium/ATP Cocktail (Cat.# 20-113)
    应用
    应用 MAP Kinase/Erk Assay Kit is designed to measure the phosphotransferase activity of MAP (mitogen-activated protein) Kinase in immunoprecipitates & column fractions.
    主要应用
    • Kinase Assay
    生物信息
    可用的分析物
    • MAP Kinase/Erk
    Entrez基因编号
    • NM_138957.2
    • NM_002745.4
    Entrez基因汇总 The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.
    基因符号
    • ERK2
    • MAPK1
    • p40
    • PRKM2
    • MAPK2
    • PRKM1
    • p42-MAPK
    • p38
    • p41
    • ERK
    • ERK-2
    • ERT1
    • P42MAPK
    • p41mapk
    UniProt编号
    • P28482
    UniProt汇总 FUNCTION: SwissProt: P28482 # Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
    COFACTOR: Magnesium (By similarity).
    SIZE: 360 amino acids; 41390 Da
    SUBUNIT: Interacts with MORG1 (By similarity). Binds to HIV-1 Nef through its SH3 domain. This interaction inhibits its tyrosine- kinase activity. Interacts with HSF4.
    DOMAIN: SwissProt: P28482 The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
    PTM: Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme.
    SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain.
    产品使用声明
    质量保证 The assay kit is designed to measure the phosphotransferase activity of MAP (mitogen-activated protein) Kinase in immunoprecipitates and column fractions. The assay kit is based on phosphorylation of a specific substrate (myelin basic protein, MBP). The phosphorylated substrate is then analyzed by immunoblot analysis, probing with a monoclonal Phospho-specific MBP antibody.
    销售限制 This product is derived from bovine source. Export of this product to certain countries may be restricted. Please contact Customer Service or your local distributor to inquire about product availability and export options.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    包装信息
    数量 1 kit

    merck millipore,默克密理博,17-191,MAP Kinase/Erk Assay Kit, non-radioactive

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