merck millipore,默克密理博,17-10062,ChIPAb+ E2F-3 - ChIP Validated Antibody and Primer Set

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merck millipore,默克密理博,17-10062,ChIPAb+ E2F-3 - ChIP Validated Antibody and Primer Set
产品名称：ChIPAb+ E2F-3 - ChIP Validated Antibody and Primer Set
产品型号：17-10062
This ChIPAb+ E2F-3 -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.

merck millipore,默克密理博,17-10062,ChIPAb+ E2F-3 - ChIP Validated Antibody and Primer Set

产品介绍

merck millipore,默克密理博,17-10062,ChIPAb+ E2F-3 - ChIP Validated Antibody and Primer Set

重要规格表

品种反应性	主要应用
R, H, M	ChIP, WB

描述
产品目录编号	17-10062
品牌系列	Upstate
商名	Upstate ChIPAb+
描述	ChIPAb+ E2F-3 - ChIP Validated Antibody and Primer Set
概述	All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction. The ChIPAb+ E2F-3 set includes the E2F-3 antibody, a negative control normal mouse IgG, and qPCR primers which amplify a 100 bp region of human CDC2 promoter. The E2F-3 and negative controls are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of E2F-3-associated chromatin.
Alternate Names	Transcription factor E2F3
背景信息	E2F transcription factors are functionally regulated by binding to Rb p110, p107, and p130. E2F 3 is regulated by complex formation with Rb p110. E2F family members bind DNA as heterodimers with members of the DP family of polypeptides.

产品信息
格式	Purified
控制	Includes normal mouse IgG and primers specific for human CDC2 coding region.
演示	Anti-E2F-3 (mouse monoclonal), . One vial containing 25 ug of protein A purified mouse IgG2a in ~180 uL of storage buffer (10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide before addition of 30% glycerol). Store at -20°C. Normal Mouse IgG. One vial containing 25 ug purified IgG in 25 uL storage buffer containing 0.1% sodium azide. Store at -20°C. ChIP Primers, human CDC2 coding region, . One vial containing 75 μL of 5 μM each primer specific for human CDC2 coding region. Store at -20°C. FOR: CGC CCT TTC CTC TTT CTT TC REV: ATC GGG TAG CCC GTA GAC TT

应用
应用	This ChIPAb+ E2F-3 -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
主要应用	Chromatin Immunoprecipitation (ChIP) Western Blotting
应用说明	Chromatin Immunoprecipitation: Sonicated chromatin prepared from HeLa S3 cells (4 X 106 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 1 µg of either Normal Mouse IgG, or 1 µg Anti-E2F-3 and the Magna ChIP™ A Kit (Cat. # 17-610). Successful immunoprecipitation of E2F-3 associated DNA fragments was verified by qPCR using ChIP Primers, human CDC2 promoter as a positive locus, and a intergenic region near human CALML5 as a negative locus. (Figure 2). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated. Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details. Western Blot Analysis: Representative lot data. Lysates from HeLa cells probed with anti-E2F-3 at 2 μg/mL. Arrow indicates E2F-3 (~58 kDa) (Figure 3).

生物信息
免疫原品种	Recombinant full-length human E2F-3 protein
表位	Unknown
宿主	Mouse
特异性	E2F-3
同种型	IgG2a
品种反应性	RatHumanMouse
Species Reactivity Note	Human and mouse. Weak reactivity with rat.
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_001940.1
Entrez基因汇总	The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F2, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner.
基因符号	DKFZp686C18211 E2F3 KIAA0075 E2F-3 MGC104598
纯化方法	Protein A purfied
UniProt编号	O00716
UniProt汇总	FUNCTION: SwissProt: O00716 # Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC- 3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-3 binds specifically to RB1 protein, in a cell-cycle dependent manner. SIZE: 465 amino acids; 49162 Da SUBUNIT: Component of the DRTF1/E2F transcription factor complex. Binds cooperatively with DP-1 to E2F sites. Interacts with retinoblastoma protein RB1 and related proteins (such as RBL1) that inhibit the E2F transactivation domain. Binds EAPP. SUBCELLULAR LOCATION: Nucleus. SIMILARITY: SwissProt: O00716 ## Belongs to the E2F/DP family.

产品使用声明
质量保证	Chromatin Immunoprecipitation: Sonicated chromatin prepared from HeLa S3 cells (4 X 106 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 1 µg of either Normal Mouse IgG, or 1 µg Anti-E2F-3 and the Magna ChIP™ A Kit (Cat. # 17-610). Successful immunoprecipitation of E2F-3 associated DNA fragments was verified by qPCR using ChIP Primers, human CDC2 promoter (Figure 1). Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品使用声明

质量保证

Chromatin Immunoprecipitation:
Sonicated chromatin prepared from HeLa S3 cells (4 X 106 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 1 µg of either Normal Mouse IgG, or 1 µg Anti-E2F-3 and the Magna ChIP™ A Kit (Cat. # 17-610).
Successful immunoprecipitation of E2F-3 associated DNA fragments was verified by qPCR using ChIP Primers, human CDC2 promoter (Figure 1).
Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.