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merck millipore,默克密理博,12-628,Calyculin A/Okadaic acid-treated A431Cell Lysate
产品名称:Calyculin A/Okadaic acid-treated A431Cell Lysate
产品型号:12-628
Cellular protein preparation from Calyculin A/Okadaic acid-treated A431 Cells (a human carcinoma cell line).
merck millipore,默克密理博,12-628,Calyculin A/Okadaic acid-treated A431Cell Lysate
- 产品介绍
重要规格表
主要应用 WB 描述 产品目录编号 12-628 品牌系列 Upstate
商名 - Upstate
描述 Calyculin A/Okadaic acid-treated A431Cell Lysate 长描述 Cellular protein preparation. Cells were serum starved overnight with serum-free media. Cells were cultured for 6 hours in the presence of a final concentration of 50nM Calyculin A (19-139) and 500nM Okadaic acid (495604). Cells were washed with ice-cold PBS and lysed in RIPA buffer. Protein concentration was determined and diluted with 2X SDS-PAGE sample loading buffer with 5% 2-Mercaptoethanol. Lysate was then boiled for 5 minutes. 产品信息 演示 100 μg in 100 μL of lysate diluted to 1mg/mL in reducing sample buffer. Concentration: 1 mg/mL. Frozen solution. 应用 应用 Cellular protein preparation from Calyculin A/Okadaic acid-treated A431 Cells (a human carcinoma cell line). 主要应用 - Western Blotting
生物信息 浓缩 1 mg/mL 品种 Human 产品使用声明 质量保证 Routinely evaluated as a positive control for some of Upstates antibodies. 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 6 months at -20°C from date of shipment. 包装信息 数量 100 µg
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