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merck millipore,默克密理博,17-10046,ChIPAb+ Histone H3 (CT) - ChIP Validated Antibody and Primer Set, rabbit monoclonal
产品名称：ChIPAb+ Histone H3 (CT) - ChIP Validated Antibody and Primer Set, rabbit monoclonal
产品型号：17-10046
This ChIPAb+ Histone H3 (C-term) -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
merck millipore,默克密理博,17-10046,ChIPAb+ Histone H3 (CT) - ChIP Validated Antibody and Primer Set, rabbit monoclonal
- 产品介绍
- merck millipore,默克密理博,17-10046,ChIPAb+ Histone H3 (CT) - ChIP Validated Antibody and Primer Set, rabbit monoclonal
重要规格表
品种反应性 主要应用 Ch, H, M, R, Yeast (S. cerevisiae) ChIP, WB 描述 产品目录编号 17-10046 品牌系列 Upstate
商名 - Upstate
- ChIPAb+
描述 ChIPAb+ Histone H3 (CT) - ChIP Validated Antibody and Primer Set, rabbit monoclonal 概述 All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
The ChIPAb+ Histone H3 (C-term) set includes the Histone H3 (C-term) antibody, a negative control supernatant (rabbit), and qPCR primers which amplify a 110 bp region of human β-Globin. The Histone H3 (C-term) and negative controls are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of Histone H3-associated chromatin.Alternate Names - histone cluster 3, H3
- H3
- Histone H3
- H3 histone family, member T
- histone 3, H3
背景信息 Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Histone proteins are highly post-translationally modified, and Histone H3 is the most extensively modified of the five histones. The term "Histone H3" alone is purposely ambiguous in that it does not distinguish between sequence variants or modification state. Histone H3 is an important protein in the emerging field of epigenetics, where its sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes. 产品信息 格式 Culture Supernatant 控制 - Includes negative control supernatant (rabbit) and primers specific for human β-Globin.
演示 Anti-Histone H3 (C-term) (rabbit monoclonal IgG). One vial containing 100 µL of cultured supernatant in 0.05% sodium azide. Store at -20°C.
Negative Control Supernatant (rabbit). One vial containing 100 µL of cultured supernatant in 0.05% sodium azide. Store at -20°C.
ChIP Primers, β-Globin.
One vial containing 75 μL of 5 μM of each primer specific for human β-Globin. Store at -20°C.
FOR: AGG ACA GGT ACG GCT GTC ATC
REV: TTT ATG CCC AGC CCT GGC TC应用 应用 This ChIPAb+ Histone H3 (C-term) -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers. 主要应用 - Chromatin Immunoprecipitation (ChIP)
- Western Blotting
应用说明 Chromatin Immunoprecipitation:
Sonicated chromatin prepared from HeLa cells (1 X 106 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 4 µL of a negative control supernatant, or 4 µL of Anti-Histone H3 (C-term) and the Magna ChIP™ A Kit (Cat. # 17-610).
Successful immunoprecipitation of Histone H3 associated DNA fragments was verified by qPCR using ChIP Primers, β-Globin as well as GAPDH promoter primers. (Please see figures). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.
Western Blot Analysis:
Representative Lot Data.
Acid extracts from colcemid treated HeLa cells were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Histone H3 (C-term) (1:1000 dilution).
Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. (Please see figures).
Western Blot Analysis:
Representative lot data.
Acid extracted proteins from HeLa cells untreated (Lane 1), colcemid treated (Lane 2). HeLa nuclear extracts (Lane 3), acid extracted HeLa cells treated with sodium butyrate (Lane 4), untreated (Lane 5) and unmodified recombinant Histone H3 (Lane 6) were resolved by electrophoresis, transferred to nitrocellulose and probed with a previous lot of anti-Histone H3 (C-term) (1:5000 dilution).
Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. (Please see figures).
生物信息 免疫原品种 KLH-conjugated, synthetic peptide corres-ponding to the C-terminus of human Histone H3. 表位 C-Terminus 宿主 Rabbit 特异性 Recognizes Histone H3 near the C-terminus. 同种型 IgG 品种反应性 ChickenHumanMouseRatYeast (S. cerevisiae) Species Reactivity Note Human, mouse, rat, yeast and chicken. Broad species cross-reactivity expected due to sequence homology. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_003484
Entrez基因汇总 Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in
eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by
RefSeq].
基因符号 - OTTHUMP00000037945
- H3.4
- H3/g
- H3/t
- H3FT
- H3T
- H3t
- MGC126886
- MGC126888
UniProt编号 - Q16695
UniProt汇总 FUNCTION: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
SUBUNIT STRUCTURE: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Expressed in testicular cells.
Developmental stage Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 By similarity.
Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription By similarity.
Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression By similarity.
Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin By similarity.
Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation By similarity.
Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin.
Ubiquitinated By similarity.
SIMILARITY:Belongs to the histone H3 family.
产品使用声明 质量保证 Chromatin Immunoprecipitation:
Sonicated chromatin prepared from HeLa cells (1 X 106 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 4 µL of either Negative Control Supernatant or 4 µL of Anti-Histone H3 (C-term) and the Magna ChIP™ A Kit (Cat. # 17-610). Successful immunoprecipitation of Histone H3 associated DNA fragments was verified by qPCR using ChIP Primers, β-Globin (Please see figures).
Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.
使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. 包装信息 数量 25 assays 包装 25 assays per set. Recommended use: ~4 μL of antibody per chromatin immunoprecipitation (dependent upon biological context).
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