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merck millipore,默克密理博,06-1364,Anti-SRp30b (SC35) Antibody
产品名称：Anti-SRp30b (SC35) Antibody
产品型号：06-1364
Anti-SRp30b (SC35) Antibody is a Rabbit Polyclonal Antibody for detection of SRp30b (SC35) also known as serine/arginine-rich splicing factor 2, Splicing factor SC35 & has been validated in WB, ICC.
merck millipore,默克密理博,06-1364,Anti-SRp30b (SC35) Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H ICC, WB Rb Purified Polyclonal Antibody 描述 产品目录编号 06-1364 描述 Anti-SRp30b (SC35) Antibody Alternate Names - serine/arginine-rich splicing factor 2
- Splicing factor SC35
- Splicing component, 35 kDa
- splicing factor, arginine/serine-rich 2
- SR splicing factor 2
背景信息 SRp30b, also known as SC35, and Splicing factor, arginine/serine-rich 2 (SFRS2) is an essential splicing factor. This protein is encoded by the SFRS2 gene and is a member of the splicing factor SR family. SRp30b is involved in pre-mRNA splicing and is found in bodies in the nucleus that are highly enriched in poly(A) RNA. These nuclear bodies are referred to as speckles, SC 35 domains, or splicing factor compartments (SFCs). The purpose of these bodies is not completely characterized. Speckles correspond largely to, if not entirely to, ultra structures called inter-chromatin granule clusters (IGCs). SRp30b is required for pre-mRNA splicing events and is highly involved in spliceosomal interaction and assembly. This SR protein is necessary for assembly of the ATP-dependent splicing complex. It is also necessary for the ATP-dependent communication between pre-mRNA and U1 and U2 snRNPs. 产品信息 格式 Purified 控制 - NIH/3T3 cells.
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-SRp30b (SC35) Antibody is a Rabbit Polyclonal Antibody for detection of SRp30b (SC35) also known as serine/arginine-rich splicing factor 2, Splicing factor SC35 & has been validated in WB, ICC. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Western Blot Analysis:
A 1:500 dilution of a representative lot of this antibody detected SRp30b in 10 µg of HeLa cell lysate.
Immunoprecipitation Analysis:
A representative lot of this antibody was used by an independent laboratory in IP. (Jiang, K., et al. (2009). Endocrinology. 150(5):2087-2097.)生物信息 免疫原品种 Linear peptide corresponding to human SRp30b. 宿主 Rabbit 品种反应性 Human Species Reactivity Note Demonstrated to react with human. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_001182356
Entrez基因汇总 The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. Two transcript variants encoding the same protein and one non-coding transcript variant have been found for this gene. In addition, a pseudogene of this gene has been found on chromosome 11. [provided by RefSeq]. 基因符号 - SRSF2
- SC-35
- PR264
- SFRS2
- SC35
- SRp30b
- SFRS2A
- Protein PR2643
纯化方法 Protein A UniProt编号 - Q01130
UniProt汇总 FUNCTION: Necessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. Can bind to beta-globin mRNA and commit it to the splicing pathway.
SUBUNIT STRUCTURE: In vitro, self-associates and binds SRSF1/SFRS1 (ASF/SF2), SNRNP70 and U2AF1 but not U2AF2. Binds SFRS12. Interacts with CCNL1 and CCNL2. Interacts with SRSF2IP/SFRS2IP. Interacts with ZRSR2/U2AF1-RS2.
SUBCELLULAR LOCATION: Nucleus.
PTM: Extensively phosphorylated on serine residues in the RS domain.
SEQUENCE SIMILARITIES: Belongs to the splicing factor SR family.
Contains 1 RRM (RNA recognition motif) domain.产品使用声明 质量保证 Evaluated by Immunocytochemistry Analysis:
A 1:500 dilution of this antibody detected SRp30b in NIH/3T3 cells.
使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µL
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