merck millipore,默克密理博,03-114,RIPAb+ Musashi 1 - RIP Validated Antibody and Primer Set, rabbit monoclonal

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merck millipore,默克密理博,03-114,RIPAb+ Musashi 1 - RIP Validated Antibody and Primer Set, rabbit monoclonal
产品名称：RIPAb+ Musashi 1 - RIP Validated Antibody and Primer Set, rabbit monoclonal
产品型号：03-114
This RIPAb+ Musashi 1 -RIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.

merck millipore,默克密理博,03-114,RIPAb+ Musashi 1 - RIP Validated Antibody and Primer Set, rabbit monoclonal

产品介绍

merck millipore,默克密理博,03-114,RIPAb+ Musashi 1 - RIP Validated Antibody and Primer Set, rabbit monoclonal

重要规格表

品种反应性	主要应用
R, H	ICC, WB, FC

描述
产品目录编号	03-114
品牌系列	Upstate
商名	Upstate RIPAb+
描述	RIPAb+ Musashi 1 - RIP Validated Antibody and Primer Set, rabbit monoclonal
概述	RIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes a negative control antibody to ensure specificity of the RIP reaction and is verified for the co-immunoprecipitation of RNA associated specifically with the immunoprecipitated RNA binding protein of interest. Where appropriate, the RIPAb+ set also includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully co-precipitating the specific RNA targets, such as messenger RNAs. The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. If a target specific assay is not provided, the RIPAb+ kit is validated using an automated microfluidics-based assay by enrichment of detectable RNA over control immunoprecipitation.
Alternate Names	musashi homolog 1 (Drosophila) Musashi (Drosophila) homolog 1 musashi 1
背景信息	In mammals, the Musashi family is important for cell fate determination, playing roles in maintenance of the stem-cell state, differentiation and tumorigenesis. Musashi-1 (also known as Msi1), is selectivley expressed in neural progenitor cells, including neural stem cells. Outside the nervous system, Musashi-1 is a selective marker for intestinal stem or early lineage cells. Musashi-1 interacts with the Notch pathway during asymmetric cell division by binding the 3' UTR of Numb. Numb is prevented from repressing Notch signaling when Musashi-1 is present.

产品信息
格式	Unpurified
控制	Includes negative control rabbit IgG antibody and control primers specific for human NUMB.
演示	Anti-Musashi 1 (Rabbit Monoclonal). One vial containing 50 μL of Rabbit Monoclonal Antibody in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Store at -20°C. Normal Rabbit IgG. One vial containing 125 μg of Rabbit IgG in 125 μL of storage buffer containing 0.05% sodium azide. Store at -20°C. RIP Primers, NUMB. One vial containing 75 μL of 5 μM of each primer specific for human NUMB. Store at -20°C. FOR: AGC CAG CCC ATA CTG CTC TA REV: CGG ACG CTC TTA GAC ACC TC

应用
应用	This RIPAb+ Musashi 1 -RIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
主要应用	Immunocytochemistry Western Blotting Flow Cytometry
应用说明	Immunoprecipitation from RIP lysate: Representative lot data. RIP lysate from SHSH-5Y cells (2 X 10E6 cell equivalents per IP) was subjected to immunoprecipitation using 0.5 µL of either a normal rabbit IgG or 0.5 µL of Anti-Musashi 1 antibody. Precipitated proteins (lane 1: rabbit IgG, lane 2: anti-Musashi 1) and SHSH-5Y whole cell lysate (lane 3) were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Musashi 1 antibody (1:2,000 dilution). Proteins were visualized using One-Step™ IP-Western kit (GenScript). Arrow indicates Musashi 1 (Please see figures). Immunocytochemistry Analysis: Representative lot data. A previous lot of this antibody was used in immunofluorescent staining of PC12 cells using Anti-Musashi 1 (Please see figures). Immunohistochemistry (Paraffin) Analysis: Representative lot data. A previous lot of this antibody was used in immunohistochemical staining of paraffin-embedded human brain using Anti-Musashi 1 (Please see figures). Western Blot Analysis: Representative lot data. SHSY5Y cell lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with Anti-Musashi 1 (1:2,000 dilution). Proteins were visualized using a Goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Musashi 1 (~39 kDa) (Please see figures). Flow Cytometry: A 1:40 dilution of a previous lot was used in flow cytometry.

生物信息
免疫原品种	Synthetic peptide corresponding to residues on the N-terminus of human Musashi-1.
表位	N-terminus
宿主	Rabbit
特异性	Recognizes Musashi 1 at the N-terminus.
同种型	IgG
品种反应性	RatHuman
Species Reactivity Note	Human and rat.
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_002433.1
Entrez基因汇总	This gene encodes a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Expression of this gene has been correlated with the grade of the malignancy and proliferative activity in gliomas and melanomas. A pseudogene for this gene is located on chromosome 11q13.
基因符号	MSI1
纯化方法	Unpurified
UniProt编号	O43347
UniProt汇总	FUNCTION: RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U1-3AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system By similarity. SUBCELLULAR LOCATION: Cytoplasm By similarity. Nucleus By similarity. TISSUE SPECIFICITY: Detected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines. DOMAIN: The first RNA recognition motif binds more strongly to RNA compared to the second one By similarity. SEQUENCE SIMILARITIES: Belongs to the Musashi family. Contains 2 RRM (RNA recognition motif) domains.

产品使用声明
质量保证	RNA Binding Protein Immunoprecipitation: RIP Lysate prepared from SHSH-5Y cells (2 X 10E7 cell equivalents per IP) were subjected to immunoprecipitation using 5 µL of either a normal rabbit IgG or 5 µL of Anti-Musashi 1 antibody and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700). Successful immunoprecipitation of Musashi 1-associated RNA was verified by qPCR using RIP Primers NUMB (Please see figures). Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品使用声明

质量保证

RNA Binding Protein Immunoprecipitation:
RIP Lysate prepared from SHSH-5Y cells (2 X 10E7 cell equivalents per IP) were subjected to immunoprecipitation using 5 µL of either a normal rabbit IgG or 5 µL of Anti-Musashi 1 antibody and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700).
Successful immunoprecipitation of Musashi 1-associated RNA was verified by qPCR using RIP Primers NUMB (Please see figures).
Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details.

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.