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品种反应性 主要应用 宿主 格式 抗体类型 H WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 06-1130 描述 Anti-RNF168 Antibody Alternate Names - RING finger protein 168
- E3 ubiquitin-protein ligase RNF168
背景信息 RNF168 is a ubiquitin ligand that binds to ubiquinated histone H2A and localizes to sites of DNA damage. RNF168-dependent chromatin modifications cause 53BP1 and BRCA1 to accumulated at the site. A mutation in RNF168 has been associated with RIDDLE syndrome, an immunodeficiency and radiosensitivity disorder. RNF168 is responsible for securing repair factors at sites of DNA damage. RNF168 has two MIU (motif interacting with ubiquitin) and one UMI (UIM- and MIU-related UBD) ubiquitin binding domains (UBDs) which are essential for proper localization of RNF168. 产品信息 格式 Affinity Purified 控制 - Jurkat cell lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-RNF168 Antibody is a Rabbit Polyclonal Antibody for detection of RNF168 also known as E3 ubiquitin-protein ligase RNF168 & has been validated in WB. 主要应用 - Western Blotting
应用说明 Immunoprecipitation Analysis: A representative lot of this antibody was used to immunoprecipitate RNF168 from whole cell lysates (Zhao, H., et al. (2014) Cell Cycle. 13(11):1–11). 生物信息 免疫原品种 Histidine-tagged recombinant protein corresponding to the C-terminus of human RNF168. 表位 C-terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes RNF168 at the C-terminus. 品种反应性 Human Species Reactivity Note Demonstrated to react with Human. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_689830
Entrez基因汇总 The complex repair response elicited by DNA double-strand breaks (DSBs) includes recruitment of several DNA repair proteins and ubiquitination of H2A-type histones (see MIM 142720). RNF168 is an E3 ubiquitin ligase critical for DSB repair (Stewart et al., 2009 [PubMed 19203578]).[supplied by OMIM]. 基因符号 - RNF168
纯化方法 Affinity purified UniProt编号 - Q8IYW5
UniProt汇总 FUNCTION: E3 ubiquitin-protein ligase required for accumulation of repair proteins to sites of DNA damage. Acts with UBE2N/UBC13 to amplify the RNF8-dependent histone ubiquitination. Recruited to sites of DNA damage at double-strand breaks (DSBs) by binding to ubiquitinated histone H2A and ubiquitinates histone H2A and H2AX, leading to amplify the RNF8-dependent H2A ubiquitination and promoting the formation of 'Lys-63'-linked ubiquitin conjugates. This leads to concentrate ubiquitinated histones H2A and H2AX at DNA lesions to the threshold required for recruitment of TP53BP1 and BRCA1.
PATHWAY: Protein modification; protein ubiquitination.
SUBUNIT STRUCTURE: Interacts with UBE2N/UBC13.
SUBCELLULAR LOCATION: Nucleus. Note: Localizes to sites of DNA damage.
DOMAIN: The MIU motifs (motif interacting with ubiquitin) mediate the interaction with ubiquitin and the localization at sites of DNA damage.
INVOLVEMENT IN DISEASE: Defects in RNF168 are the cause of Riddle syndrome (RIDDLES) [MIM:611943]. Riddle syndrome is characterized by increased radiosensitivity, immunodeficiency, mild motor control and learning difficulties, facial dysmorphism, and short stature. Defects are probably due to impaired localization of TP53BP1 and BRCA1 at DNA lesions.
SEQUENCE SIMILARITIES: Belongs to the RNF168 family.
Contains 1 RING-type zinc finger.
SEQUENCE CAUTION: The sequence BAB70801.1 differs from that shown. Reason: Erroneous initiation.
The sequence BAC04060.1 differs from that shown. Reason: Erroneous initiation.产品使用声明 质量保证 Evaluated by Western Blot in Jurkat cell lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected RNF168 on 10 µg of Jurkat cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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下一件merck millipore产品：merck millipore,默克密理博,AB2984,Anti-Integrin beta-3 Antibody
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