merck millipore,默克密理博,16-282,Anti-Phosphotyrosine Antibody, clone 4G10, Magnetic Bead Conjugate

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Merck Millipore 默克密理愽

品种反应性	主要应用	宿主	格式	抗体类型
A	IP	M	Purified	Monoclonal Antibody

描述
产品目录编号	16-282
描述	Anti-Phosphotyrosine Antibody, clone 4G10®, Magnetic Bead Conjugate
背景信息	Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. Some of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine.).The advent of anti-phosphotyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.

产品信息
格式	Purified
控制	NIH3T3 whole cell lysate stimulated with 50 ng/mL PDGF (5 minutes)
演示	20% slurry; 1xPBS, 0.05% sodium azide. Sufficient for 10 IPs.

应用
应用	Detect Phosphotyrosine using this Anti-Phosphotyrosine Antibody, clone 4G10®, Magnetic Bead Conjugate validated for use in IP.
主要应用	Immunoprecipitation

生物信息
免疫原品种	Phosphotyramine-KLH
克隆	clone 4G10®
宿主	Mouse
特异性	This antibody recognizes tyrosine-phosphorylated proteins from all species
同种型	IgG2bκ
品种反应性	All
Species Reactivity Note	All
抗体类型	Monoclonal Antibody
纯化方法	Protein G-Sepharose Chromatography

产品使用声明
质量保证	100 μg of NIH3T3 whole cell lysate stimulated with 100 ng/mL PDGF (10 minutes) was immunoprecipitated (IP) using either anti-mouse IgG (Cat. No. 12-371) (lane 1), or Anti-Phosphotyrosine, Magnetic Bead Conjugate (Lane 2).
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at 2-8ºC from date of receipt. Note: DO NOT FREEZE

包装信息
数量	200 µL

merck millipore,默克密理博,16-282,Anti-Phosphotyrosine Antibody, clone 4G10, Magnetic Bead Conjugate

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