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主要应用 Entrez基因编号 Uni Prot编号 HMT NM_199141.1 Q86X55 描述 产品目录编号 14-1048 Replaces 14-575 品牌系列 Upstate
商名 - Upstate
描述 Human recombinant CARM1 概述 Human recombinant CARM1 with an N-terminal FLAG tag expressed in FreeStyle 293-F cells. 应用 应用 Human recombinant CARM1 with an N-terminal FLAG tag expressed in FreeStyle 293-F cells. 主要应用 - Histone Methyltransferase Assay (HMT)
生物信息 纯度 Varies by lot; see lot-specific datasheet for information on each lot. 来源 Human 特异活性 For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. Entrez基因编号 - NM_199141.1
Entrez基因汇总 Protein arginine N-methyltransferases, such as CARM1, catalyze the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. Protein arginine methylation has been implicated in signal transduction, metabolism of nascent pre-RNA, and transcriptional activation (Frankel et al., 2002 [PubMed 11724789]).[supplied by OMIM] 基因符号 - PRMT4
- CARM1
UniProt编号 - Q86X55
UniProt汇总 FUNCTION: SwissProt: Q86X55 # Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' and activates transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at 'Arg-2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs.
SIZE: 585 amino acids; 63460 Da
SUBUNIT: Homodimer (Probable). Interacts with the C-terminus of NCOA2/GRIP1, NCO3/ACTR and NCOA1/SRC1. Part of a complex consisting of CARM1, EP300/P300 and NCOA2/GRIP1. Interacts with FLII, TP53, myogenic factor MEF2, EP300/P300, CREBBP and CTNNB1. Interacts with SNRPC (By similarity). Interacts with NR1H4. Interacts with RELA. Interacts with HTLV-1 Tax-1.
SUBCELLULAR LOCATION: Nucleus. Cytoplasm.
TISSUE SPECIFICITY: Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia.
SIMILARITY: SwissProt: Q86X55 ## Belongs to the protein arginine N-methyltransferase family.
MISCELLANEOUS: Methylation of H3-R17 by CARM1 is stimulated by preacetylation of H3-K18/H3-K23 by EP300 and blocked by citrullination of H3-R17 by PADI4 (By similarity).产品使用声明 质量保证 routinely evaluated by methylation of histone H3 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 6 months at -80°C. 包装信息 数量 10 µg
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