merck millipore,默克密理愽
  • merck millipore,默克密理博,AB5541,Anti-Glial Fibrillary Acidic Protein Antibody

  • merck millipore,默克密理博,AB5541,Anti-Glial Fibrillary Acidic Protein Antibody

    产品名称：Anti-Glial Fibrillary Acidic Protein Antibody
    产品型号：AB5541
    This Anti-Glial Fibrillary Acidic Protein Antibody is validated for use in IC, IH, IH(P), WB for the detection of GFAP.

    merck millipore,默克密理博,AB5541,Anti-Glial Fibrillary Acidic Protein Antibody

  • 产品介绍
  • merck millipore,默克密理博,AB5541,Anti-Glial Fibrillary Acidic Protein Antibody

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    Po, H, M, R, BWB, ICC, IHCChAffinity PurifiedPolyclonal Antibody
    描述
    产品目录编号 AB5541
    Replaces 04-1031; 04-1062
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-Glial Fibrillary Acidic Protein Antibody
    Alternate Names
    • GFAP
    背景信息 Glial fibrillary acidic protein (GFAP) is a class-III intermediate filament. Type III intermediate filaments contain three domains, the most conserved of which is the rod domain. The specific sequence for this region of the protein may differ between the different intermediate filament genes for type III proteins, but the structure of the protein is highly conserved. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes.
    产品信息
    格式 Affinity Purified
    控制
    • Astrocytes, astrocytomas, neural stem cells.
    演示 Purified chicken polyclonal in buffer containing PBS containing 5 mM sodium azide.
    应用
    应用 This Anti-Glial Fibrillary Acidic Protein Antibody is validated for use in IC, IH, IH(P), WB for the detection of GFAP.
    主要应用
    • Western Blotting
    • Immunocytochemistry
    • Immunohistochemistry
    应用说明 Immunohistochemistry(paraffin):
    Representative images from a previous lot. Pictures of Optimal Staining With Citrate Buffer Epitope Retrieval: Human Brain.

    Immunocytochemistry:
    1:200-1:1,000 on cells in tissue culture fixed for one minute in 3.7% formalin and one minute in -20°C methanol.

    Western blot:
    1:100-1:500 using ECL. Recognizes a band of 55 kDa. May also react with a band of 50 kDa.

    Immunohistochemistry:
    1:100-1:500 on frozen tissue sections. Suggested fixative is 4% paraformaldehyde.

    Optimal working dilutions must be determined by the end user.

    STAINING PATTERN:

    The antibody stains sharply defined cytoplasmic filaments of astroglia cells in tissue culture. Stains processes of astrocytes in sections of brain tissues.
    生物信息
    免疫原品种 Purified bovine GFAP.
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Chicken
    特异性 Glial fibrillary acidic protein (GFAP). Reacts with both native and recombinant protein.
    品种反应性 PigHumanMouseRatBovine
    Species Reactivity Note Human, rat, and mouse. Expected to cross-react with bovine and porcine. Other species have not yet been tested.
    抗体类型 Polyclonal Antibody
    Entrez基因编号
    • NM_002055.2
    Entrez基因汇总 This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant has been described, but its full length sequence has not been determined.
    基因符号
    • FLJ45472
    • GFAP
    纯化方法 Immunoaffinity purified
    UniProt编号
    • P14136
    UniProt汇总 FUNCTION: SwissProt: P14136 # GFAP, a class-III intermediate filament, is a cell- specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
    SIZE: 432 amino acids; 49880 Da
    SUBUNIT: Isoform 3 interacts with N-terminus of PSEN1.
    SUBCELLULAR LOCATION: Cytoplasm. Note=Associated with intermediate filaments.
    DISEASE: SwissProt: P14136 # Defects in GFAP are a cause of Alexander disease [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
    SIMILARITY: SwissProt: P14136 ## Belongs to the intermediate filament family. ...
    产品使用声明
    质量保证 Routinely evaluated by Western Blot on Mouse Brain lysates.

    Western Blot Analysis: 1:1000 dilution of this lot detected glial fibrillary acidic protein on 10 μg of Mouse Brain lysates.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at -20°C in undiluted aliquots from date of receipt. Do not store in a self-defrosting freezer.

    Handling Recommendations:
    Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
    包装信息
    数量 50 µL

    merck millipore,默克密理博,AB5541,Anti-Glial Fibrillary Acidic Protein Antibody

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