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merck millipore,默克密理博,09-310,Anti-phospho-EGFR (Tyr1069) Antibody
产品名称：Anti-phospho-EGFR (Tyr1069) Antibody
产品型号：09-310
This Anti-phospho-EGFR (Tyr1069) Antibody is validated for use in ELISA, IC, WB for the detection of phospho-EGFR (Tyr1069).
merck millipore,默克密理博,09-310,Anti-phospho-EGFR (Tyr1069) Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, M, Po, Pm, Eq WB, ELISA, ICC Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 09-310 Replaces 04-339 描述 Anti-phospho-EGFR (Tyr1069) Antibody Alternate Names - epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
- Receptor tyrosine-protein kinase ErbB-1
- avian erythroblastic leukemia viral (v-erb-b) oncogene<br />homolog
- cell growth inhibiting protein 40
- cell proliferation-inducing protein 61
- epidermal growth factor receptor
- epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b) oncogene homolog)
- epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog
背景信息 EGFR (Epidermal growth factor receptor, ErbB-1) is a receptor tyrosine kinase (RTK) that is one of four members of the EGFR/ErbB family of receptor tyrosine kinases. EGFR plays a key role in the regulation of essential normal cellular processes and in the pathophysiology of hyperproliferative diseases such as cancer. Activation of the EGFR signaling pathway has been linked with increased cell proliferation, angiogenesis, metastasis and decreased apoptosis. Upon ligand binding, EGFR undergoes a transition from an inactive monomeric form to an active dimer. In addition to forming homodimers, EGFR may pair with another member of the ErbB receptor family, such as ErbB2/Her2/neu, to create an activated heterodimer. EGFR dimerization stimulates its intrinsic intracellular protein-tyrosine kinase activity that leads to trans- and auto-phosphorylation of tyrosine residues within the cytoplasmic domain of the receptor tyrosine kinase of five tyrosine (Y) residues on sites Y992, Y1045, Y1068, Y1148 and Y1173 located on the C-terminal domain of EGFR. This autophosphorylation elicits downstream activation of several signal transduction cascades, principally the MAPK, Akt and JNK pathways through Ras, PI3 Kinase, and PLC pathways that ultimately leads to the activation of the various pathways downstream. Tyr1069 is phosphorylated during activation. 产品信息 格式 Affinity Purified 控制 - Untreated and EGF treated A431 lysates
演示 Antigen affinity purified rabbit polyclonal in 0.1M Tris-Glycine (pH 7.4) 150mM NaCl, with 0.05% sodium azide. 应用 应用 This Anti-phospho-EGFR (Tyr1069) Antibody is validated for use in ELISA, IC, WB for the detection of phospho-EGFR (Tyr1069). 主要应用 - Western Blotting
- ELISA
- Immunocytochemistry
应用说明 Immunofluroescent Immunocytochemistry: 生物信息 免疫原品种 Tyrosine-phosphorylated epidermal growth factor receptor. 表位 Tyr1069 (cytoplasmic domain)
宿主 Rabbit 特异性 Reacts strongly and specifically with Tyr1069 phosphorylated EGFR 品种反应性 RatHumanMousePigPrimateHorse Species Reactivity Note Tested in Human. Expected to react with mouse, rat, primate, horse and pig based on 100% sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_005228.3
Entrez基因汇总 The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq]
基因符号 - EC 2.7.10.1
- ERBB
- ERBB1
- HER1
- PIG61
- mENA
修改 - Phosphorylation
纯化方法 Antigen affinity purified UniProt编号 - P00533
UniProt汇总 FUNCTION: Receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with C-SRC and CTNNB1/beta-catenin. Ref.32
Isoform 2/truncated isoform may act as an antagonist. Ref.32
Catalytic activity ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
SUBUNIT: Binds RIPK1. CBL interacts with the autophosphorylated C-terminal tail of the EGF receptor. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. The autophosphorylated form interacts with PIK3C2B, maybe indirectly. Interacts with PELP1. Binds MUC1. Ref.32 Ref.26 Ref.30 Ref.31 Ref.36
SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein.
Isoform 2: Secreted.
TISSUE SPECIFICITY: Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers. Ref.39
PTM: Phosphorylation of Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Ref.17 Ref.25 Ref.29 Ref.33 Ref.34 Ref.35 Ref.37 Ref.38 Ref.40 Ref.41 Ref.42 Ref.43
Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.
DISEASE: Defects in EGFR are associated with lung cancer [MIM:211980].
Miscellaneous Binding of EGF to the receptor leads to dimerization, internalization of the EGF-receptor complex, induction of the tyrosine kinase activity, stimulation of cell DNA synthesis, and cell proliferation.
SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain.
产品使用声明 质量保证 Routinely evaluated by Western Blot on untreated and EGF treated A431 lysates.
Western Blot Analysis:
1:1,000 dilution of this antibody detected Tyr1069 phosphorylated EGFR on 10 g of EGF treated A431 lysates.
使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8ºC in undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
包装信息 数量 100 µL
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